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Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Filter Antibodies: (3371)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| α-actinin | Primary |
Santa Cruz Biotechnology sc-17829 |
Unmodified |
Citations
(1) Datasheet |
Mouse Monoclonal | NCI-H1975 | Size | Not_Stated | Not_Stated |
| α-Cardiac Actin | Primary |
R&D Systems MAB9308 |
alpha-Cardiac Actin | Datasheet | Mouse Monoclonal | Human, mouse, and rat heart tissue | Size | 2 µg/mL | 50 |
| α-Nucleocapsid | Primary |
Novus Biologicals NB100-56683 |
SARS Nucleocapsid Protein | Datasheet | Rabbit Polyclonal | Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) | Size | 25 mg/mL | 60 |
| α-Synuclein | Primary |
Cell Signaling Technology 2642 |
(α) alpha-Synuclein | Rabbit Polyclonal | Size | 1:25 | 21 | ||
| α-Synuclein | Primary |
Cell Signaling Technology 2647 |
(α) alpha-Synuclein (Syn204) | Mouse Monoclonal | Spinal cord 3 ug/ul | Size | 1:10 | 22 | |
| α-Synuclein | Primary |
Cell Signaling Technology 2647 |
(α) alpha-Synuclein (Syn204) | Mouse Monoclonal | Spinal cord 3 ug/uL | Size | 1:10 | 22 | |
| α-Synuclein, Phospho | Primary |
Cell Signaling Technology 23706 |
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) | Rabbit Monoclonal | Spinal cord 3 ug/ul | Size | 1:250 | 23 | |
| α-Synuclein, Phospho | Primary |
Cell Signaling Technology 93706 |
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) | Rabbit Monoclonal | Spinal cord 3 ug/uL | Size | 1:250 | 23 | |
| α-Tubulin | Primary |
MilliporeSigma T8203 |
alpha Tubulin | Mouse Monoclonal | Transfected HeLa, Human Primary Fibroblast Lysates | Size | 1:100 | ||
| β-Actin | Primary |
Cell Signaling Technology 4967 |
β-Actin | Datasheet | Rabbit Polyclonal | HeLa | Size | 1:10 - 1:50 | 48 |
| β-Actin | Primary |
MilliporeSigma A5411 |
(β) beta-actin | Mouse Monoclonal | Rat Brain Lysate (0.2 mg/mL) | Size | 1:400 | 48 | |
| β-actin | Primary |
MilliporeSigma A5441 |
(β) beta-actin | Mouse Monoclonal | Rat Brain Lysate (0.2 mg/mL) | Size | 1:400 | 48 | |
| β-Actin | Primary |
GeneTex GTX101794 |
Actin Gamma 1 | Rabbit Polyclonal | Human Stomach Smooth Muscles | Size | 1:100 | 44 | |
| β-Actin | Primary |
MilliporeSigma A5316 |
beta-actin | Mouse Monoclonal | Transfected HeLa, Human Primary Fibroblast Lysates | Size | 1:100 | ||
| β-Actin (13E5) | Primary |
Cell Signaling Technology 4970 |
β-Actin (13E5) | Datasheet | Rabbit Monoclonal | HeLa | Size | 1:10 - 1:50 | 48 |
| β-Actin (8H10D10) | Primary |
Cell Signaling Technology 3700 |
β-Actin (8H10D10) | Datasheet | Mouse Monoclonal | HeLa | Size | 1:10 - 1:50 | 49 |
| β-Actin (D6A8) | Primary |
Cell Signaling Technology 8457 |
Unmodified |
Citations
(4) Datasheet |
Rabbit Monoclonal | CHO | Size | 1:50 | Not_Stated |
| β-Catenin | Primary |
Cell Signaling Technology 8480 |
(β) beta-Catenin | Rabbit Monoclonal | NIH-3T3, A172, U87MG, U251 | Size | 1:250 | 90 | |
| β-catenin | Primary |
Cell Signaling Technology 8480 |
Datasheet | Rabbit Monoclonal | HL60 Cell Lysates | Size | 1:25, 1:100, 1:400 | 85 | |
| β-Catenin | Primary |
Cell Signaling Technology 9562 |
(β) beta-Catenin | Rabbit Polyclonal | HeLa | Size | 1:50 | ||
| β-Catenin (D10A8) | Primary |
Cell Signaling Technology 8480 |
Unmodified |
Citations
(2) Datasheet |
Rabbit Monoclonal | A-172 | Size | 1:250 | Not_Stated |
| β-Catenin, Phospho | Primary |
Cell Signaling Technology 2009 |
(β) beta-Catenin, Phospho S33/37 | Rabbit Polyclonal | NIH-3T3, A172, U87MG, U251 | Size | 1:50 | 92 | |
| β-Catenin, Phospho | Primary |
Cell Signaling Technology 9561 |
(β) beta-Catenin, S33/37/T41 | Rabbit Polyclonal | NIH-3T3, A172, U87MG, U251 | Size | 1:50 | 92 | |
| β-Tubulin | Primary |
Cell Signaling Technology 2125 |
Unmodified |
Citations
(9) Datasheet |
Rabbit Monoclonal | Brain | Size | 1:2000 | Not_Stated |
| β-Tubulin | Primary |
LI-COR 926-42211 |
Unmodified |
Citations
(9) Datasheet |
Rabbit Monoclonal | 4T1 | Size | 1:100 | Not_Stated |
| β-Tubulin | Primary |
Abcam ab15568 |
Human (β) beta Tubulin aa 400 to the C-terminus | Rabbit Polyclonal | Mouse embryonic fibroblasts | Size | 1:50 | ||
| β-Tubulin | Primary |
MilliporeSigma T4026 |
(β) beta-Tubulin | Mouse Monoclonal | Huh7.5.1 | Size | |||
| 14-3-3 γ | Primary |
Novus Biologicals NB100-406 |
14-3-3 gamma (γ) | Datasheet | Mouse Monoclonal | HeLa lysate 1.0 mg/ml | Size | 1:12.5 | 36 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27202 |
14-3-3 gamma (γ) | Datasheet | Rabbit polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:50 | 33 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27370 |
14-3-3 gamma (γ) | Datasheet | Rabbit Polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:10 | 34 |
| 14-3-3 ζ | Primary |
R&D Systems MAB2669 |
14-3-3 zeta (ζ) | Datasheet | Mouse Monoclonal | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 |
| 15-PGDH/HPGD | Primary |
R&D Systems AF5660 |
15-PGDH/HPGD | Datasheet | Goat Polyclonal | Human liver tissue | Size | 10 µg/mL | 34 |
| 15-PGDH/HPGD | Primary |
Novus Biologicals NB200-179 |
15-PGDH/HPGD | Datasheet | Rabbit polyclonal | Lovo lysate 0.5 mg/ml | Size | 1:500 | 34 |
| 17 beta-HSD1/HSD17B1 | Primary |
R&D Systems MAB7178 |
17 beta-HSD1/HSD17B1 | Datasheet | Mouse Monoclonal | Human placenta tissue | Size | 2.5 µg/mL | 40 |
| 4-Hydroxynonenal | Primary |
R&D Systems MAB3249 |
4-Hydroxynonenal | Datasheet | Mouse Monoclonal | HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) | Size | 5 µg/mL | |
| 4E-BP1, phospho | Primary |
Cell Signaling Technology 9455 |
Phospho 4E-BP1 (T70) | Rabbit polyclonal | Human Liver Tissue | Charge | |||
| 4EBP1 | Primary |
R&D Systems AF3227 |
4EBP1 | Datasheet | Goat Polyclonal | HeLa human cervical epithelial carcinoma cell line | Size | 5 µg/mL | 26 |
| 4EBP1 | Primary |
MilliporeSigma 07-1416 |
4EBP1 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Epitomics 1557-1 |
4EBP1 | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 4923 |
Non-p4EBP1 (Thr46) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 9452 |
4EBP1 | Rabbit polyclonal | Caco2 cell lysates | Size | 1:50 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 9644 |
4EBP1 | Rabbit monoclonal | MCF7 lysed in Bicine/CHAPS | Size | 1:50-1:100 | 28-32 | |
| 4EBP1 | Primary |
Cell Signaling Technology 9644 |
4EBP1 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:200-1:1000 | ||
| 4EBP1, phospho | Primary |
Epitomics 2334-1 |
p4EBP1 (Thr45) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 2855 |
p4EBP1 (Thr37/46) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9451 |
p4EBP1 (Ser65) | Rabbit polyclonal | K562 -/+ Gleevec lysed in Bicine CHAPS | Size | 1:50 | 27-31 | |
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9451 |
p4EBP1 (Ser65) | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9456 |
p4EBP1 (Ser65) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9459 |
p4EBP1(Thr37/46) | Rabbit polyclonal | K562 -/+ Gleevec lysed in Bicine CHAPS | Size | 1:50 | 27-31 | |
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9459 |
p4EBP1 (Thr37/46) | Rabbit polyclonal | MCF7 lysed in Bicine/CHAPS | Charge | 1:50-1:100 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9459 |
p4EBP1 (Thr37/46) | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP2 | Primary |
Cell Signaling Technology 2845 |
4EBP2 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:100 | ||
| 4HPPD | Primary |
Proteintech 17004-1-AP |
4-HPPD | Rabbit polyclonal | recombinant protein expression in E. coli | Size | 1:50 | 52 | |
| 5'-Nucleotidase/CD73 | Primary |
R&D Systems AF5795 |
5'-Nucleotidase/CD73 | Datasheet | Sheep Polyclonal | U266 human myeloma cell line | Size | 10 µg/mL | 86 |
| 5-HT2AR | Primary |
LSBio LS-C172270 |
5-HT2AR | Rabbit polyclonal | Rat cortex | Size | 1:500 | ||
| 5-HT7 | Primary |
Novus Biologicals NB100-56352 |
5-Hydroxytryptamine receptor 7 | Datasheet | Rabbit Polyclonal | Rat Brain Lysate | Size | 20 µg/mL | 59 |
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58748 |
5-Lipoxygenase | Datasheet | Rabbit polyclonal | Human Lung lysate 0.5 mg/ml | Size | 50 µg/mL | |
| 5-Lipoxygenase | Primary |
Novus Biologicals NB110-58749 |
5-Lipoxygenase | Datasheet | Rabbit polyclonal | Human Lung lysate 0.5 mg/ml | Size | 10 µg/mL | |
| 5HT1D | Primary |
Novus Biologicals NB100-56349 |
5HT1D | Datasheet | Rabbit polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:10 | |
| 5HT2C (5-hydroxytryptamine Receptor 2C)/Serotonin Receptor 2C | Primary |
Santa Cruz Biotechnology sc-17797 |
5-HT2CR | Mouse | Rat brain fractions | Size | 1:50 | 55 | |
| 5HT3A | Primary |
Novus Biologicals NB100-56351 |
5HT3A | Datasheet | Rabbit polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:50 | 65 |
| 5T4 | Primary |
R&D Systems AF4975 |
5T4 | Datasheet | Sheep Polyclonal | human placenta | Size | 50 µg/ml | 90 |
| 6-His Epitope Tag | Primary |
Novus Biologicals NB100-64768 |
6-His Epitope Tag | Datasheet | Mouse monoclonal | rhEDG6 CHO S Tx lysate 0.1 mg/ml | Size | 1:250 | |
| 6X-His tag-HRP | Primary |
Thermo Fisher Scientific MA1-21315-HRP |
Tag | Mouse Monoclonal | Size | 1:50, 1:100 | |||
| A RAF | Primary |
Abcam ab200653 |
A RAF | Rabbit Monoclonal | A375 cell line | Size | 1:50 | ||
| a-Smooth Muscle Actin (D4K9N) | Primary |
Cell Signaling Technology 19245 |
a-Smooth Muscle Actin (D4K9N) | Datasheet | Rabbit Monoclonal | Human Colon | Size | 1:10 - 1:50 | 49 |
| A. aeolicus BPL/BioID2 | Primary |
Novus Biologicals NBP2-59940 |
A. aeolicus BPL/BioID2 Antibody (SS QD1) | Datasheet | Mouse Monoclonal | Size | 94 | ||
| A20 | Primary |
Origene TA318419 |
A20 | Rabbit | Caco cell lysate | Size | |||
| A20 (TNFa-induced protein 1 member 3) | Primary |
Origene TA307167 |
A20 | Rabbit monoclonal | Caco2 cell lysates | Size | 1:50 | ||
| A20/TNFAIP3 | Primary |
Novus Biologicals NBP1-77533 |
A20/TNFAIP3 | Datasheet | Mouse monoclonal | Jurkat lysate 0.05 mg/ml | Size | 1:100 | 102 |
| A20/TNFAIP3 | Primary |
Cell Signaling Technology 5630 |
Unmodified |
Citations
(1) Datasheet |
Rabbit Monoclonal | TMD8 | Size | Not_Stated | Not_Stated |
| A2BP1 | Primary |
Novus Biologicals NBP2-13169 |
A2BP1 | Datasheet | Mouse monoclonal | Human Brain lysate 0.5 mg/ml | Size | 1:20 | 54 |
| A33 | Primary |
R&D Systems MAB30802 |
A33 | Datasheet | Mouse Monoclonal | HT-29, COLO 205 | Size | 50 µg/ml | 40 |
| A33 | Primary |
R&D Systems MAB30803 |
A33 | Datasheet | Rabbit Monoclonal | Human Colon | Size | 50 µg/ml | 40 |
| AAV VP1 | Primary |
PROGEN 61056 |
AAV VP1 | Mouse Monoclonal | AAV2 purified from HEK293 cell lysate | Size | 1:50 | 104 | |
| AAV VP1/VP2 | Primary |
PROGEN 61057 |
AAV VP1/VP2 | Mouse Monoclonal | AAV2 purified from HEK293 cell lysate | Size | 1:50 | 104/82 | |
| AAV VP1/VP2/VP3 | Primary |
PROGEN 61058 |
AAV VP1/VP2/VP3 | Mouse Monoclonal | AAV2 purified from HEK293 cell lysate | Size | 1:50 | 104/82/64 | |
| ABCA1 | Primary |
Novus Biologicals NB400-105 |
ABCA1 | Datasheet | Rabbit polyclonal | Charge | 1:50 | ||
| ABCA1 | Primary |
Abcam ab18180 |
ABCA1 | Mouse Monoclonal | Mouse liver lysate | Size | 1:50 | 270 | |
| ABCF2 | Primary |
Novus Biologicals NB400-115 |
ABCF2 | Datasheet | Rabbit polyclonal | HeLa lysate 0.05 mg/ml | Size | 1:1000 | 77 |
| ABCG1 | Primary |
Abcam ab52617 |
ABCG1 | Rabbit monoclonal | Mouse liver lysate | Size | 1:50 | 110 (mouse) -116 (human) | |
| ABCG2 | Primary |
Novus Biologicals NBP1-59749 |
ABCG2 | Datasheet | Rabbit polyclonal | HepG2 lysate 0.5 mg/ml | Size | 1:50 | 72 |
| ABCG2 | Primary |
Abcam ab24115 |
ABCG2 | Rat monoclonal | 3T3, MCF7, H460 | Size | 1:20 | 100 | |
| Abhd5 | Primary |
Novus Biologicals NB110-41576 |
Abhd5 | Datasheet | Rabbit polyclonal | MCF-7 lysate 0.5 mg/ml | Size | 1:1000 | 55 |
| ACADL | Primary |
Novus Biologicals NBP1-54936 |
ACADL Antibody | Datasheet | Rabbit Polyclonal | Rat/mouse liver | Size | 1:50 | 48 |
| ACADL | Primary |
Aviva Systems Biology arp33855_p050 |
ACADL | Rabbit polyclonal | Rat and mouse liver | Size | 1:50 | 47 | |
| ACC | Primary |
Cell Signaling Technology 3662 |
Acetyl CoA Carboxylase | Rabbit Polyclonal | gt1-7 | Size | 1:50 | 207 | |
| ACE-2 | Primary |
R&D Systems AF3437 |
ACE-2 | Datasheet | Goat Polyclonal | Mouse kidney tissue | Size | 2.5 µg/mL | 141 |
| ACE-2 | Primary |
R&D Systems AF933 |
ACE-2 | Datasheet | Goat Polyclonal | Human kidney tissue | Size | 10 µg/mL | 155 |
| ACE-2 | Primary |
R&D Systems AF933 |
ACE-2 | Datasheet | Goat Polyclonal | Recombinant Human ACE-2 Protein, CF (R&D Systems PN 933-ZN-010) | Size | 1:50 | 158 |
| ACE/CD143 | Primary |
R&D Systems AF1513 |
ACE/CD143 | Datasheet | Goat Polyclonal | Mouse lung tissue | Size | 10 µg/mL | 218 |
| Acetyl-CoA carboxylase | Primary |
Cell Signaling Technology 3661 |
Ser79 | Rabbit Polyclonal | HEK293T | Size | 1:200 | ||
| Acetyl-CoA Carboxylase | Primary |
Cell Signaling Technology 3676 |
AcetylCoA | Rabbit monoclonal | Hela, K562 | Size | 1:200 | 230 | |
| Acetyl-CoA Carboxylase (ACC), phospho | Primary |
Cell Signaling Technology 3661 |
pACC (Ser 79) | Rabbit Polyclonal | HEK 293 | Size | 1:200 | ||
| Acetylated Lysine | Primary |
Cell Signaling Technology 9441 |
Acetylated-Lysine | Rabbit Polyclonal | Huh7.5 | Charge | 1:50 | ||
| Acrp30 | Primary (biotinylated) |
R&D Systems BAF1065 |
Acrp30 | Datasheet | Goat | Human Serum | Size | 1:50 | 40 |
| ACSF2 | Primary |
Novus Biologicals NBP2-47558 |
ACSF2 Antibody | Datasheet | Rabbit Polyclonal | Size | |||
| ACSL1 | Primary |
Novus Biologicals NB110-99585 |
ACSL1 | Datasheet | Rabbit polyclonal | HepG2 lysate 0.5 mg/ml | Size | 1:100 | 65 |
| ACSL3 | Primary |
Novus Biologicals NBP2-15252 |
ACSL3 Antibody | Datasheet | Rabbit Polyclonal | Mouse brain (hippocampus) tissue | Size | ||
| ACSL4 | Primary |
Santa Cruz Biotechnology sc-365230 |
ACSL4 | Mouse monoclonal | Rat and Mouse Liver | Size | 1:50 | 66 |
