Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution▲ | kDa on Simple Western |
---|---|---|---|---|---|---|---|---|---|
β-Tubulin | Primary |
MilliporeSigma T4026 |
(β) beta-Tubulin | Mouse Monoclonal | Huh7.5.1 | Size | |||
4E-BP1, phospho | Primary |
Cell Signaling Technology 9455 |
Phospho 4E-BP1 (T70) | Rabbit polyclonal | Human Liver Tissue | Charge | |||
A. aeolicus BPL/BioID2 | Primary |
Novus Biologicals NBP2-59940 |
A. aeolicus BPL/BioID2 Antibody (SS QD1) | Datasheet | Mouse Monoclonal | Size | 94 | ||
A20 | Primary |
Origene TA318419 |
A20 | Rabbit | Caco cell lysate | Size | |||
ACSF2 | Primary |
Novus Biologicals NBP2-47558 |
ACSF2 Antibody | Datasheet | Rabbit Polyclonal | Size | |||
ACSL3 | Primary |
Novus Biologicals NBP2-15252 |
ACSL3 Antibody | Datasheet | Rabbit Polyclonal | Mouse brain (hippocampus) tissue | Size | ||
ACSL5 | Primary |
Novus Biologicals NBP1-59645 |
ACSL5 Antibody | Datasheet | Rabbit Polyclonal | Mouse liver lysate | Size | ||
ADAM10 | Primary |
Cell Signaling Technology 14194 |
ADAM10 | Rabbit Polyclonal | Size | 60 and 100 | |||
Akt | Primary |
Cell Signaling Technology 9272 |
Unmodified |
Citations
(20) Datasheet |
Rabbit Polyclonal | Size | |||
AKT, phospho | Primary |
Cell Signaling Technology 13038 |
Phospho AKT (T308) | Rabbit monoclonal | Human Liver Tissue | Charge | |||
alcohol dehydrogenase 5 | Primary |
Novus Biologicals NBP2-49350 |
alcohol dehydrogenase 5 Antibody | Datasheet | Rabbit Polyclonal | Size | |||
alpha-Fetoprotein/AFP | Primary |
Novus Biologicals NB110-7961 |
alpha-Fetoprotein/AFP | Datasheet | Mouse Monoclonal | Size | |||
AMPK alpha, phospho | Primary |
Cell Signaling Technology 2531 |
AMPKα, Phospho (Thr172) | Rabbit Polyclonal | Size | 63 | |||
Androgen Receptor | Primary |
Abcam ab52615 |
Androgen Receptor | Rabbit Monoclonal | Size | 120 | |||
Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 805-035-180 |
Anti-Goat IgG (H+L) HRP Conjugated | Bovine Polyclonal | Charge | ||||
Anti-Rabbit IgG (H+L) HRP | Secondary |
MilliporeSigma A0545 |
Anti-Rabbit IgG (H+L) HRP Conjugated Minimal Cross Reactivity to Human | Goat Polyclonal | Charge | ||||
Anti-Rabbit IgG HRP | Secondary |
Cell Signaling Technology 7074 |
Anti-Rabbit IgG HRP | Rabbit polyclonal | Human Liver Tissue | Charge | |||
ASIC2 | Primary |
LSBio LS-C93915 |
ACCN1 | Mouse Polyclonal | mouse fibroblast cell line | Size | 54 | ||
ATF-3 (D2Y5W) | Primary |
Cell Signaling Technology 33593 |
Citations
(1) Datasheet |
Rabbit Monoclonal | Size | ||||
ATP Synthase | Primary |
Abcam ab96655 |
ATP Synthase | Rabbit Polyclonal | Human bladder tumor cells (T24 cells) and human skin fibroblasts (CAFs) | Size | |||
ATR, phospho | Primary |
Cell Signaling Technology 2853 |
ATR, Phospho (Ser428) | Rabbit Polyclonal | Size | 300 | |||
BDNF | Primary |
Novus Biologicals NB120-10505 |
BDNF Antibody (35928.11) | Datasheet | Mouse Monoclonal | Size | |||
BEST1 | Primary |
LAgen Laboratories 016-Best1-01 |
BEST1 | Rabbit polyclonal | iPSC-RPE | Size | |||
Beta actin | Primary |
Santa Cruz Biotechnology sc-47778 |
Beta actin | Mouse monoclonal | Size | ||||
beta Adducin | Primary |
Novus Biologicals NBP2-33974 |
beta Adducin Antibody | Datasheet | Rabbit Polyclonal | Size | |||
beta II Tubulin A | Primary |
Novus Biologicals NB120-11308 |
beta II Tubulin A Antibody (TUB 2.1) | Datasheet | Mouse Monoclonal | Huh7.5.1 | Size | ||
BID | Primary |
Cell Signaling Technology 2002 |
BID | Rabbit polyclonal | Human Liver Tissue | Charge | |||
Bim | Primary |
Cell Signaling Technology 2819 |
Bim | Rabbit polyclonal | HEL, KG1a, Nomo-1, MOLM-14, HL60, K562 | Charge | |||
Bim | Primary |
MilliporeSigma ab17003 |
Bim | Rabbit polyclonal | HEL, KG1a, Nomo-1, MOLM-14, HL60, K562 | Charge | |||
Bim (C34C5) | Primary |
Cell Signaling Technology 2933 |
Citations
(1) Datasheet |
Mouse Monoclonal | Size | ||||
Bip | Primary |
Cell Signaling Technology 3183 |
Bip | Rabbit Monoclonal | CaCo 2BB | Size | 65 | ||
BLK, Phospho | Primary |
Abcam ab192670 |
Phospho Tyr501 | Rabbit Polyclonal | Mouse splenocytes +/- LCWE treatment | Size | |||
Bovine Serum Albumin | Primary |
Novus Biologicals NB100-2195 |
Bovine Serum Albumin Antibody (3G3A2) | Datasheet | Mouse Monoclonal | Size | |||
C2CD2 | Primary |
Novus Biologicals NBP2-33941 |
C2CD2 Antibody | Datasheet | Rabbit Polyclonal | Size | |||
C2orf42 | Primary |
Novus Biologicals NBP2-48620 |
C2orf42 Antibody | Datasheet | Rabbit Polyclonal | Size | |||
Cathepsin E | Primary |
Novus Biologicals NBP2-33868 |
Cathepsin E Antibody | Datasheet | Rabbit Polyclonal | Size | |||
CD36 | Primary |
Novus Biologicals NB400-145 |
CD36 Antibody | Datasheet | Rabbit Polyclonal | Human adipose lysate | Size | ||
CD63 | Primary |
Novus Biologicals NBP2-67425 |
CD63 Antibody (SY21-02) | Datasheet | Rabbit Monoclonal | Size | |||
CDK19 | Primary |
MilliporeSigma HPA007053 |
CDK19 | Rabbit Polyclonal | HT29 shRNA treated | Size | 70 | ||
CDK8 | Primary |
Cell Signaling Technology 4106 |
CDK8 | Rabbit Polyclonal | HT29 shRNA treated | Size | 64 | ||
cGAS | Primary |
Santa Cruz Biotechnology sc515777 |
cGAS | Mouse Monoclonal | Size | 58 | |||
CHD7 | Primary |
Cell Signaling Technology 6505 |
CHD7 (N-Terminus) | Rabbit Monoclonal | Neural Crest Cells, Neuroepithelial Cells | Size | |||
cMet | Primary |
Abcam ab51067 |
cMet | Rabbit monoclonal | Human brain microvascular endothelial cells | Size | |||
COX-1 | Primary |
Novus Biologicals NBP1-85500 |
COX-1 Antibody | Datasheet | Rabbit Polyclonal | Mouse tail vein lysates | Size | 70 | |
CPI17 alpha | Primary |
Novus Biologicals NBP2-37897 |
CPI17 alpha Antibody | Datasheet | Rabbit Polyclonal | Size | |||
CRALBP | Primary |
Novus Biologicals NB100-74392 |
CRALBP | Datasheet | Mouse monoclonal | iPSC-RPE | Size | ||
CRISPR-Cas9 | Primary |
Novus Biologicals NBP2-36440 |
CRISPR-Cas9 Antibody (7A9-3A3) - N-Terminus | Datasheet | Mouse Monoclonal | HeLa Cas9 lysate and Hela WT lysate | Size | 158 | |
CRISPR-Cas9 | Primary |
Novus Biologicals NBP2-52398 |
CRISPR-Cas9 Antibody (6G12) - C-terminus | Datasheet | Mouse Monoclonal | HeLa Cas9 lysate and Hela WT lysate | Size | 158 | |
CXCL16 | Primary |
Abcam ab101404 |
CXCL16 | Rabbit polyclonal | Macrophages | Size | |||
Cyclophilin | Primary |
Abcam ab133504 |
Cyclophilin | Rabbit Monoclonal | Human bladder tumor cells (T24 cells) and human skin fibroblasts (CAFs) | Size |