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Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Filter Antibodies: (3371)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation▲ | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| BChE | Primary |
Santa Cruz Biotechnology sc-377403 |
Specific for aa 85-119 near the N-terminus of BChE | Mouse Monoclonal | Yeast | - | 1:20 | 106 | |
| COX4 | Primary |
Novus Biologicals NBP2-43540 |
COX4 | Datasheet | Mouse monoclonal | Titrated to saturation using various models | - | 6 µg/mL | |
| CSF1R | Primary |
Cell Signaling Technology 3152 |
M-CSF Receptor | Rabbit Polyclonal | Mouse Macrophages, Mouse Myeloid Leukemia Cell Line (RIPA lysis buffer: 214, 165, 59 ; active motif nuclear extact kit whole-cell lysis buffer: 189, 59 ) | - | 1:25 | 59, 165, 189, 214 | |
| Donkey Anti-Mouse AlexFluor® 647 | Secondary |
Thermo Fisher Scientific A-31571 |
Anti-Mouse IgG (H+L); Jess Only | Donkey Polyclonal | - | 1:100 | |||
| FCRN | primary |
Novus Biologicals NBP1-89127 |
FCRN | Datasheet | Rabbit Polyclonal | hepatocyte | - | 1:50 | 64 |
| GAPDH | Primary |
Abcam ab8245 |
GAPDH | Mouse monoclonal | - | 1:50 | 40 | ||
| GluR1 | Primary |
MilliporeSigma AB1504 |
Glutamate receptor 1 | Rabbit Polyclonal | Mouse brain lysate | - | 1:17 | 127 | |
| Hemagglutinin 2 | Primary |
Pharma Foods International HA2#16 |
HA2 | Chicken monoclonal | Viral particles in chicken eggs | - | 1.8 µg/mL | 103 | |
| IA-2/PTPRN | Primary |
R&D Systems MAB7906 |
IA-2/PTPRN | Datasheet | Mouse Monoclonal | Human brain (hypothalamus) tissue | - | 10 µg/mL | 78 |
| 4E-BP1, phospho | Primary |
Cell Signaling Technology 9455 |
Phospho 4E-BP1 (T70) | Rabbit polyclonal | Human Liver Tissue | Charge | |||
| 4EBP1 | Primary |
MilliporeSigma 07-1416 |
4EBP1 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Epitomics 1557-1 |
4EBP1 | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 4923 |
Non-p4EBP1 (Thr46) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 9644 |
4EBP1 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:200-1:1000 | ||
| 4EBP1, phospho | Primary |
Epitomics 2334-1 |
p4EBP1 (Thr45) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 2855 |
p4EBP1 (Thr37/46) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9451 |
p4EBP1 (Ser65) | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9456 |
p4EBP1 (Ser65) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9459 |
p4EBP1 (Thr37/46) | Rabbit polyclonal | MCF7 lysed in Bicine/CHAPS | Charge | 1:50-1:100 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9459 |
p4EBP1 (Thr37/46) | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP2 | Primary |
Cell Signaling Technology 2845 |
4EBP2 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:100 | ||
| ABCA1 | Primary |
Novus Biologicals NB400-105 |
ABCA1 | Datasheet | Rabbit polyclonal | Charge | 1:50 | ||
| Acetylated Lysine | Primary |
Cell Signaling Technology 9441 |
Acetylated-Lysine | Rabbit Polyclonal | Huh7.5 | Charge | 1:50 | ||
| AKT | Primary |
Cell Signaling Technology 2920 |
AKT1/2/3 | Mouse monoclonal | MEF and NHLF | Charge | 1:100 | ||
| AKT | Primary |
Cell Signaling Technology 4691 |
AKT |
Citations
(5) Datasheet |
Rabbit monoclonal | MCF7 lysed in Bicine/CHAPS | Charge | 1:50-1:100 | |
| AKT | Primary |
Cell Signaling Technology 9272 |
AKT1/2/3 | Rabbit polyclonal | EGF stimulated MCF-7 | Charge | 1:50 | ||
| AKT, phospho | Primary |
Cell Signaling Technology 13038 |
Phospho AKT (T308) | Rabbit monoclonal | Human Liver Tissue | Charge | |||
| AKT, phospho | Primary |
Epitomics 2118 |
pAKT (Ser473) | Rabbit monoclonal | EGF stimulated MCF-7 | Charge | 1:50 | ||
| AKT, phospho | Primary |
Cell Signaling Technology 2965 |
pAKT (Thr 308) | Rabbit monoclonal | MCF7 lysed in Bicine/CHAPS | Charge | 1:50-1:100 | ||
| AKT, phospho | Primary |
Cell Signaling Technology 4056 |
pAKT (Thr308) | Rabbit monoclonal | EGF stimulated MCF-7 | Charge | 1:50 | ||
| AKT, phospho | Primary |
Cell Signaling Technology 4060 |
pAKT (Ser473) | Rabbit monoclonal | A549 | Charge | 1:50 | ||
| AKT, phospho | Primary |
Cell Signaling Technology 9267 |
pAKT (Ser450) | Rabbit polyclonal | Cancer cell lines | Charge | 1:50 | ||
| AKT, phospho | Primary |
Cell Signaling Technology 9271 |
pAKT (Ser473) | Rabbit polyclonal | EGF stimulated MCF-7 | Charge | 1:100 | ||
| AKT1 | Primary |
MilliporeSigma 05-796 |
AKT1 | Rabbit monoclonal | EGF stimulated MCF-7 | Charge | 1:50 | ||
| AKT1 | Primary |
Cell Signaling Technology 2938 |
AKT1 | Rabbit monoclonal | MCF7 lysed in Bicine/CHAPS | Charge | 1:50-1:100 | ||
| AKT1 | Primary |
BD Biosciences BD-610861 |
AKT1 | Mouse monoclonal | Cancer cell lines | Charge | 1:50 | ||
| Akt1 | Primary |
Santa Cruz Biotechnology sc-1618 |
Unmodified |
Citations
(1) Datasheet |
Goat Polyclonal | ES2,JHOC-5,RMG-I | Charge | Not_Stated | Not_Stated |
| Akt1, phospho | Primary |
MilliporeSigma 05-669 |
pAkt1 (Ser473) | Mouse monoclonal | Tumor cells, cell line | Charge | 1:25-1:50 | ||
| Akt1/2/3 | Primary |
Santa Cruz Biotechnology sc-8312 |
Unmodified |
Citations
(7) Datasheet |
Rabbit Polyclonal | HL-60,MOLM-14,MV4-11,OCI-AML3, Bone marrow | Charge | 1:1000 | Not_Stated |
| Akt1/2/3 | Primary |
Santa Cruz Biotechnology sc-8312 |
Unmodified |
Citations
(7) Datasheet |
Rabbit Polyclonal | Null | Charge | Not_Stated | Not_Stated |
| AKT2 | Primary |
Cell Signaling Technology 3063 |
AKT2 | Rabbit monoclonal | Many | Charge | 1:50 | ||
| Akt2 (D6G4) | Primary |
Cell Signaling Technology 3063 |
Unmodified |
Citations
(4) Datasheet |
Rabbit Monoclonal | N1E-115, Brain | Charge | Not_Stated | |
| AKT3 | Primary |
MilliporeSigma 07-383 |
AKT3 | Rabbit polyclonal | EGF stimulated MCF-7 | Charge | 1:50 | ||
| Anti-Goat IgG (H+L) Biotin-SP | Secondary |
Jackson ImmunoResearch 305-065-045 |
Anti-Goat IgG (H+L) Biotin-SP Conjugated Minimal Cross Reactivity | Goat Polyclonal | Charge | 1:1000 | |||
| Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 705-035-003 |
Anti-Goat IgG (H+L) HRP Conjugated | Donkey Polyclonal | Charge | 1:250 | |||
| Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 705-035-147 |
Anti-Goat IgG (H+L) HRP Conjugated Minimal Cross Reactivity | Donkey Polyclonal | Charge | 1:100-1:300 | |||
| Anti-Goat IgG (H+L) HRP | Secondary |
Jackson ImmunoResearch 805-035-180 |
Anti-Goat IgG (H+L) HRP Conjugated | Bovine Polyclonal | Charge | ||||
| Anti-goat-IgG-HRP | Secondary |
Thermo Fisher Scientific 31400 |
Charge | 1:100 | |||||
| Anti-goat-IgG-HRP | Secondary |
Zymax 81-1620 |
Charge | 1:100-1:300 | |||||
| Anti-Human IgG (H+L) Biotin-SP | Secondary |
Jackson ImmunoResearch 109-065-003 |
Anti-Human IgG (H+L) Biotin-SP Conjugated | Goat Polyclonal | Charge | 1:1000 |
