Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

  • R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
  • Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
  • Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated and backed by Cell Signaling Technology (CST)

Simple Western Validated Antibodies

Filter Antibodies: (3371)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species▲ Sample Separation Dilution kDa on Simple Western
Anti-bird-IgG-HRP Secondary Bethyl Laboratories
A140-110P 		Anti-bird-IgG-HRP Size 1:250
Anti-chicken-IgG-HRP Secondary KPL
14-24-06 		Anti-chicken-IgG-HRP Size 1:300
Anti-goat-IgG-HRP Secondary Thermo Fisher Scientific
31400 		Charge 1:100
Anti-goat-IgG-HRP Secondary Zymax
81-1620 		Charge 1:100-1:300
Anti-goat-IgG-HRP Secondary Invitrogen Antibodies
87-9363 		Size 1:100-1:300
GST-HRP Primary GE
RPN 1236 		Tag Size 1:50-1:200 Depends on the recombinant protein
His -HRP penta Primary Qiagen
34460 		Tag Size 1:50-1:200 Depends on the recombinant protein
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
805-035-180 		Anti-Goat IgG (H+L) HRP Conjugated Bovine Polyclonal Charge
BDNF, Biotinylated Primary R&D Systems
BAF248 		BDNF, Biotinylated Datasheet Chicken recombinant protein spiked into Human Serum Size 1:50 19
TGF-B1 Primary R&D Systems
BAF240 		TGF-B1 Datasheet Chicken recombinant protein spiked into Human Serum Size 1:50 14
Hemagglutinin 2 Primary Pharma Foods International
HA2#16 		HA2 Chicken monoclonal Viral particles in chicken eggs - 1.8 µg/mL 103
alpha-Fetoprotein/AFP Primary R&D Systems
AF1369 		alpha-Fetoprotein/AFP Datasheet Chicken Polyclonal HepG2 human hepatocellular carcinoma cell line Size 2 µg/mL 70
Anti Sheep-HRP Secondary R&D Systems
HAF016 		Datasheet Donkey Polyclonal Size 1:50
Anti-Donkey IgG HRP Secondary R&D Systems
HAF109 		IgG (H+L Chain) Datasheet Donkey Polyclonal Detection of Goat IgG Size 1:50
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
705-035-003 		Anti-Goat IgG (H+L) HRP Conjugated Donkey Polyclonal Charge 1:250
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
705-035-147 		Anti-Goat IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Charge 1:100-1:300
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
705-035-147 		Anti-Goat IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Size 1:100-1:300
Anti-Mouse IgG (H+L) HRP Secondary Jackson ImmunoResearch
715-035-150 		Anti-Mouse IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Colorectal Cancer (CRC) tissue Charge 1:300
Anti-Mouse-IgG-HRP Secondary R&D Systems
HAF018 		Datasheet Donkey Polyclonal Size 1:100
Anti-Rabbit IgG (H+L) HRP Secondary Novus Biologicals
NB7185 		Rabbit IgG (H+L) Pre-adsorbed, HRP Conjugated Datasheet Donkey Polyclonal Size 1:50
Anti-Rabbit IgG (H+L) HRP Secondary Jackson ImmunoResearch
711-035-152 		Rabbit IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Colorectal Cancer (CRC) tissue Charge 1:300
Anti-Rat IgG (H+L) HRP Secondary Jackson ImmunoResearch
712-035-153 		Anti-Rat IgG (H+L) HRP Conjugated Donkey Polyclonal Size 1:100-1:300
Anti-Sheep IgG (H+L) HRP Secondary Jackson ImmunoResearch
713-036-147 		F(ab')2 Fragment Donkey Anti-Sheep IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Size 1:100
Anti-Sheep IgG (H+L) HRP Secondary MilliporeSigma
A3415 		Anti-Sheep IgG (H+L) HRP Conjugated Donkey Polyclonal Size 1:100
Donkey Anti-Mouse AlexFluor® 647 Secondary Thermo Fisher Scientific
A-31571 		Anti-Mouse IgG (H+L); Jess Only Donkey Polyclonal - 1:100
Donkey Anti-Rabbit AlexaFluor® 647 Secondary Thermo Fisher Scientific
A-31573 		Anti-Rabbit IgG (H+L); Jess Only Donkey Polyclonal Size 1:100
Donkey Anti-Rabbit HRP Secondary Thermo Fisher Scientific
31458 		Anti-Rabbit IgG (H+L) Donkey Polyclonal Size 1:50
Acrp30 Primary (biotinylated) R&D Systems
BAF1065 		Acrp30 Datasheet Goat Human Serum Size 1:50 40
G-CSF Primary (biotinylated) R&D Systems
BAF214 		G-CSF Datasheet Goat recombinant protein spiked into Human Serum Size 1:100 17
GDF-15 Primary (biotinylated) R&D Systems
BAF940 		GDF-15 Datasheet Goat recombinant protein spiked into Human Serum Size 1:50 14
IFN-y Primary R&D Systems
BAF285 		IFN-y Datasheet Goat recombinant protein spiked into Human Serum Size 1:100 23
IGF-1 Primary R&D Systems
BAF291 		IGF-1 Datasheet Goat recombinant protein spiked into Human Serum Size 1:10 5
IL-10 Primary R&D Systems
AF-217-NA 		IL-10 Datasheet Goat CM-PBMC + PHA Size 10 µg/mL
IL-1a Primary R&D Systems
BAF200 		IL-1a Datasheet Goat recombinant protein spiked into Human Serum Size 1:50 22
IL-1b Primary (biotinylated) R&D Systems
AF-201-NA 		IL-1b Datasheet Goat THP-1 + PMA/LPS Size 1 µg/mL
IL-1b Primary R&D Systems
BAF201 		IL-1b Datasheet Goat recombinant protein spiked into Human Serum Size 1:100 23
IL-1ra Primary R&D Systems
BAF280 		IL-1ra Datasheet Goat recombinant protein spiked into Human Serum Size 1:300 23
IL-2 Primary R&D Systems
BAF202 		IL-2 Datasheet Goat recombinant protein spiked into Human Serum Size 1:100 19
IL-6 Primary R&D Systems
AF-206-NA 		IL-6 Datasheet Goat CM- Monocytes + LPS Size 5 µg/mL
IL-6 Primary R&D Systems
BAF206 		IL-6 Datasheet Goat recombinant protein spiked into Human Serum Size 1:50 28
IL-8 Primary R&D Systems
BAF208 		IL-8 Datasheet Goat recombinant protein spiked into Human Serum Size 1:100 8
IP-10 Primary R&D Systems
BAF266 		IP-10 Datasheet Goat recombinant protein spiked into Human Serum Size 1:250 11
Leptin Primary R&D Systems
BAF398 		Leptin Datasheet Goat recombinant protein spiked into Human Serum Size 1:100 17
MCP-1 Primary R&D Systems
BAF279 		MCP-1 Datasheet Goat recombinant protein spiked into Human Serum Size 1:50 12
MMP-9 Primary R&D Systems
BAF911 		MMP-9 Datasheet Goat recombinant protein spiked into Human Serum Size 1:300 146
Myoglobin Primary Bethyl Laboratories
A150-103A 		Myoglobin Goat HeLa lysate spiked with myoglobin Size 1:100 20
TARC Primary R&D Systems
BAF364 		TARC Datasheet Goat recombinant protein spiked into Human Serum Size 1:250 5
TNF-a Primary R&D Systems
BAF210 		TNF-a Datasheet Goat recombinant protein spiked into Human Serum Size 1:50 22
TNF-alpha primary R&D Systems
AF-210-NA 		TNF-alpha Datasheet Goat CM - THP-1 + LPS Size 5 µg/mL
VEGF R1 Primary R&D Systems
BAF321 		VEGF R1 Datasheet Goat recombinant protein spiked into Human Serum Size 1:300 164
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