Simple Western - An Automated Immunoassay Analyzer

Table of Contents

• How Simple Western Capillary Immunoassays Work
• Available Molecular Weight Ranges & Detection Reagents
• New to Simple Western? We're Here to Help

How Simple Western Capillary Electrophoresis Immunoassays Work

Simple Western™ automated immunoassays take place in a capillary. A plate that contains all the required reagents is first prepared with as little as 3 µL of sample per well and placed in Jess™, Abby™, Peggy Sue™, or Sally Sue™. Approximately 40 nL of sample is loaded into the capillary and the proteins are separated by size as they migrate through a stacking and separation matrix. The separated proteins are then covalently bound to the capillary wall via a proprietary, photoactivated capture chemistry, replacing and eliminating the need to transfer protein to a membrane. Target proteins are identified using a primary antibody and detected using either an HRP-conjugated secondary antibody and chemiluminescent substrate or a fluorescently labeled secondary antibody. The resulting signal is detected and quantitated using Compass for Simple Western software.

• What Does Sized-Based Assay Data Look Like?

ReThink, ReUse, RePlex

RePlex™ on Jess and Abby feature allows you to run two sequential immunoassays within the same capillary to give you rich protein characterization data in a single detection channel.

Stellar Fluorescence Detection Modules

Stellar™ NIR/IR Modules on Jess lead the industry in fluorescence detection sensitivity for Western blotting workflows!