Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.
If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.
Single-Cell Western Validated Antibodies
Validated Target | Host Species | Vendor / Cat #▲ | Sample | Concentration |
---|---|---|---|---|
β-tubulin* | Rabbit |
Abcam ab6046 |
Murine pancreatic cancer cells | 1:40 |
Albumin | Mouse |
Abcam 10241 |
Hepatocyte co-culture | 100 µg/mL |
Atg7* | Rabbit |
Abcam ab133528 |
HeLa cells | 100 µg/mL |
ATP1A3 | Mouse |
Abcam ab2826 |
Live primary human cortical neurons | 100 µg/mL |
Blasticidin | Rabbit |
Abcam ab38307 |
Transfected HEK293T cells | 100 µg/mL |
Cas9 | Mouse |
Abcam ab191468 |
Mouse lymphocytes | 1:10 |
CD90 | Mouse |
Abcam ab23894 |
Cholangiocarcinoma cells | 100 µg/mL |
Collagen I | Mouse |
Abcam ab6308 |
(unpublished) | 100 µg/mL |
CPEB4 | Rabbit |
Abcam ab1974928 |
(unpublished) | 100 µg/mL |
CPT1A | Mouse |
Abcam ab128568 |
(unpublished) | 100 µg/mL |
Cyclin A2 | Rabbit |
Abcam ab181591 |
(unpublished) | 100 µg/mL |
Cytokeratin 8* | Mouse |
Abcam ab9023 |
Breast cancer cells | 100 µg/mL |
DDX4 | Rabbit |
Abcam ab13840 |
(unpublished) | 100 µg/mL |
EGR1 | Rabbit |
Abcam ab133695 |
Treated LNCap cells | 1:20 |
EpCAM | Rabbit |
Abcam ab213500 |
(unpublished) | 100 µg/mL |
ERa | Rabbit |
Abcam ab16660 |
MCF-7 cells | 1:10 |
Etv5 | Rabbit |
Abcam ab156221 |
Pedx3-sorted cells | 100 µg/mL |
FoxL1 | Rabbit |
Abcam ab95286 |
A549 cells | 1:10 |
GFP* | Goat |
Abcam ab6673 |
Mouse dendrites | 100 µg/mL |
Glucagon | Rabbit |
Abcam ab108426 |
(unpublished) | 100 µg/mL |
GRP75 | Mouse |
Abcam ab82591 |
U373 MG cells | 1:10 |
H3K27me3 | Mouse |
Abcam ab205729 |
Human brain nuclei | 100 µg/mL |
H3K27me3 | Mouse |
Abcam ab6002 |
Treated KARPAS cells | 100 µg/mL |
Her2 | Mouse |
Abcam ab16901 |
BT474 | |
HMGCS2* | Rabbit |
Abcam ab137043 |
(unpublished) | 100 µg/mL |
Iba1 | Goat |
Abcam ab48004 |
Dissociated mouse brain | 100 µg/mL |
IGFBP2 | Rabbit |
Abcam ab91404 |
(unpublished) | 100 µg/mL |
IL-18 | Rabbit |
Abcam ab207324 |
(unpublished) | 1:10 |
IRF5 | Goat |
Abcam ab2932 |
THP1 cells | 100 µg/mL |
LANA | Rat |
Abcam ab4103 |
Treated B-cells | 100 µg/mL |
Lin28b | Rabbit |
Abcam ab191881 |
K562 cells | |
LRRK2 | Rabbit |
Abcam ab133474 |
Dissociated mouse brain | 100 µg/mL |
mCherry | Rabbit |
Abcam ab167453 |
Mouse dendrites | 100 µg/mL |
MMP14 | Rabbit |
Abcam ab51074 |
Treated MDA-MB-231 | 100 µg/mL |
N-Ras* | Goat |
Abcam ab77392 |
Patient-derived bladder cancer cells implanted in nude mouse | 1:10 |
NEF | Mouse |
Abcam ab42358 |
(unpublished) | 1:5 |
Nkx2.1 | Rabbit |
Abcam ab7613 |
FACS sorted iPSCs | 100 µg/mL |
Olig2 (O2) | Rabbit |
Abcam ab136253 |
Differentiated Oligodendrocyte sample | 1:10 |
Ovalbumin | Rabbit |
Abcam ab1221 |
Murine sarcoma cells | 1:10 |
P-glycoprotein | Rabbit |
Abcam ab170904 |
PBMCs | 1:10 |
P-glycoprotein* | Rabbit |
Abcam ab168337 |
Treated U373 MG cells | 100 µg/mL |
p53 | Mouse |
Abcam ab1101 |
HeLa cells | 100 µg/mL |
pGSK3β | Rabbit |
Abcam ab75814 |
(unpublished) | 100 µg/mL |
pIRF3* | Rabbit |
Abcam 76493 |
THP1 cells | 100 µg/mL |
PKC δ | Rabbit |
Abcam ab182126 |
Treated LNCap cells | 1:80 |
PKCα | Rabbit |
Abcam ab32376 |
Treated LNCap cells | 1:10 |
pPKCd (Ser299) | Rabbit |
Abcam ab133456 |
Treated LNCap cells | 1:60 |
pSMAD3* | Rabbit |
Abcam ab52903 |
Treated HT1080 cells | 100 µg/mL |
PU.1 | Rabbit |
Abcam ab76543 |
THP1 cells | 100 µg/mL |
RFP | Mouse |
Abcam ab65856 |
HEK293T cells | 100 µg/mL |