Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.
If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.
Single-Cell Western Validated Antibodies
Validated Target | Host Species | Vendor / Cat # | Sample | Concentration▲ |
---|---|---|---|---|
alpha-actinin | Rabbit |
Cell Signaling Technology 6487 |
BT474 | |
Cytokeratin 19 | Rabbit |
GeneTex GTX112666 |
normal human mammary basal cells | |
FOXP3 | Goat |
R&D Systems BAF3240 |
Mouse T-regs | |
GAPDH | Goat |
Novus Biologicals NB300-320 |
Jurkat cells | |
Her2 | Mouse |
Abcam ab16901 |
BT474 | |
Her2 | Mouse |
ThermoFisher Scientific BMS120FI |
BT474 | |
Histone H3 | Rabbit |
Novus Biologicals NB500-171 |
Jurkat cells | |
Histone H3 dimethyl lys 79 | Rabbit |
Novus Biologicals NBP2-59194 |
||
Lin28b | Rabbit |
Abcam ab191881 |
K562 cells | |
phospho-S6-ribosomal | Rabbit |
Cell Signaling Technology 5364 |
BT474 | |
protein | species |
vendor product_code |
sample | |
S6 ribosomal protein | Mouse |
Cell Signaling Technology 2317 |
BT474 | |
STAT1 | Mouse |
ThermoFisher Scientific AHO0832 |
Treated Jurkat cells | |
IL-1beta | Goat |
R&D Systems AF-401-NA |
GM-CSF derived BMDCs | 10 µg/mL |
PABPC4 | Rabbit |
Novus Biologicals NB100-74593 |
Human adult bone marrow CD34+ HSPCs | 10 µg/mL |
α-actinin | Goat |
R&D Systems AF8279 |
KPC cells | 100 µg/mL |
α-actinin4 | Mouse |
Santa Cruz Biotechnology sc-390205 |
HeLa cells | 100 µg/mL |
βIII-tubulin (neuronal) | Mouse |
Sigma Aldrich T8578 |
Human iPSCs | 100 µg/mL |
Actin | Mouse |
Developmental Studies Hybridoma Bank 224-236-1 |
Dictyostelium discoideum | 100 µg/mL |
Albumin | Mouse |
Abcam 10241 |
Hepatocyte co-culture | 100 µg/mL |
Atg7 | Mouse |
R&D Systems MAB6608 |
HeLa cells | 100 µg/mL |
Atg7* | Rabbit |
Abcam ab133528 |
HeLa cells | 100 µg/mL |
ATM | Mouse |
Novus Biologicals NB100-309 |
(unpublished) | 100 µg/mL |
ATP1A3 | Mouse |
Abcam ab2826 |
Live primary human cortical neurons | 100 µg/mL |
ATP1A3/Sodium Potassium ATPase Alpha 3 | Mouse Monoclonal |
Novus Biologicals NB300-540 |
Live primary human cortical neurons | 100 µg/mL |
betaIII-tubulin | Mouse |
R&D Systems MAB1195 |
neural differentiated iPSCs | 100 µg/mL |
Blasticidin | Rabbit |
Abcam ab38307 |
Transfected HEK293T cells | 100 µg/mL |
CCR7 | Rabbit |
Novus Biologicals NBP2-58753 |
Jurkat cells | 100 µg/mL |
CD-80 (B7-1) | Goat |
R&D Systems AF140 |
Dendritic cells | 100 µg/mL |
CD209 (DC-SIGN) | Sheep |
R&D Systems AF161 |
Dendritic cells | 100 µg/mL |
CD25 | Goat |
R&D Systems AF-223-NA |
Tregs | 100 µg/mL |
CD29 | Goat |
R&D Systems AF2405 |
KPC cells | 100 µg/mL |
CD45 | Mouse |
BD Biosciences 555480 |
PBMCs | 100 µg/mL |
CD56 (NCAM-1) | Goat |
R&D Systems AF2408 |
Differentiated NK cells | 100 µg/mL |
CD68 | Rat |
Novus Biologicals NBP2-33337 |
RAW264.7 cells | 100 µg/mL |
CD68/SR-D1 | Rat |
Novus Biologicals NBP2-80656 |
RAW264.7 cells | 100 µg/mL |
CD81 | Mouse |
Novus Biologicals NBP2-53318 |
Gli36 cells | 100 µg/mL |
CD81* | Mouse |
Novus Biologicals NBP1-44861 |
Gli36 cells | 100 µg/mL |
CD83 | Goat |
R&D Systems AF2044 |
Dendritic cells | 100 µg/mL |
CD86 (B7-2) | Goat |
R&D Systems AF-141-NA |
Dendritic cells | 100 µg/mL |
CD90 | Mouse |
Abcam ab23894 |
Cholangiocarcinoma cells | 100 µg/mL |
Collagen I | Mouse |
Abcam ab6308 |
(unpublished) | 100 µg/mL |
CPEB4 | Rabbit |
Abcam ab1974928 |
(unpublished) | 100 µg/mL |
CPT1A | Mouse |
Abcam ab128568 |
(unpublished) | 100 µg/mL |
CRBN | Rabbit |
Novus Biologicals NBP1-91810 |
HeLa cells | 100 µg/mL |
CYBRD1 | Rabbit |
Genetex GTX104793 |
Red blood cells | 100 µg/mL |
Cyclin A2 | Rabbit |
Abcam ab181591 |
(unpublished) | 100 µg/mL |
Cyclin D1 | Rabbit |
R&D Systems AF4196 |
MCF-7 cells | 100 µg/mL |
Cyclophilin B | Goat |
R&D Systems AF5410 |
HEK293T cells | 100 µg/mL |
Cytochrome b reductase 1 | Rabbit |
Novus Biologicals NBP2-16075 |
Red blood cells | 100 µg/mL |