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Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies


Filter Antibodies: (394)
Validated Target Host Species Vendor / Cat # Sample Concentration▲
alpha-actinin Rabbit Cell Signaling Technology
6487
BT474
Cytokeratin 19 Rabbit GeneTex
GTX112666
normal human mammary basal cells
FOXP3 Goat R&D Systems
BAF3240
Mouse T-regs
GAPDH Goat Novus Biologicals
NB300-320
Jurkat cells
Her2 Mouse Abcam
ab16901
BT474
Her2 Mouse ThermoFisher Scientific
BMS120FI
BT474
Histone H3 Rabbit Novus Biologicals
NB500-171
Jurkat cells
Histone H3 dimethyl lys 79 Rabbit Novus Biologicals
NBP2-59194
Lin28b Rabbit Abcam
ab191881
K562 cells
phospho-S6-ribosomal Rabbit Cell Signaling Technology
5364
BT474
protein species vendor
product_code
sample
S6 ribosomal protein Mouse Cell Signaling Technology
2317
BT474
STAT1 Mouse ThermoFisher Scientific
AHO0832
Treated Jurkat cells
IL-1beta Goat R&D Systems
AF-401-NA
GM-CSF derived BMDCs 10 µg/mL
PABPC4 Rabbit Novus Biologicals
NB100-74593
Human adult bone marrow CD34+ HSPCs  10 µg/mL
α-actinin Goat R&D Systems
AF8279
KPC cells 100 µg/mL
α-actinin4 Mouse Santa Cruz Biotechnology
sc-390205
HeLa cells 100 µg/mL
βIII-tubulin (neuronal) Mouse Sigma Aldrich
T8578
Human iPSCs 100 µg/mL
Actin Mouse Developmental Studies Hybridoma Bank
224-236-1
Dictyostelium discoideum 100 µg/mL
Albumin Mouse Abcam
10241
Hepatocyte co-culture 100 µg/mL
Atg7 Mouse R&D Systems
MAB6608
HeLa cells 100 µg/mL
Atg7* Rabbit Abcam
ab133528
HeLa cells 100 µg/mL
ATM Mouse Novus Biologicals
NB100-309
(unpublished) 100 µg/mL
ATP1A3 Mouse Abcam
ab2826
Live primary human cortical neurons 100 µg/mL
ATP1A3/Sodium Potassium ATPase Alpha 3 Mouse Monoclonal Novus Biologicals
NB300-540
Live primary human cortical neurons 100 µg/mL
betaIII-tubulin Mouse R&D Systems
MAB1195
neural differentiated iPSCs 100 µg/mL
Blasticidin Rabbit Abcam
ab38307
Transfected HEK293T cells 100 µg/mL
CCR7 Rabbit Novus Biologicals
NBP2-58753
Jurkat cells 100 µg/mL
CD-80 (B7-1) Goat R&D Systems
AF140
Dendritic cells 100 µg/mL
CD209 (DC-SIGN) Sheep R&D Systems
AF161
Dendritic cells 100 µg/mL
CD25 Goat R&D Systems
AF-223-NA
Tregs 100 µg/mL
CD29 Goat R&D Systems
AF2405
KPC cells 100 µg/mL
CD45 Mouse BD Biosciences
555480
PBMCs 100 µg/mL
CD56 (NCAM-1) Goat R&D Systems
AF2408
Differentiated NK cells 100 µg/mL
CD68 Rat Novus Biologicals
NBP2-33337
RAW264.7 cells 100 µg/mL
CD68/SR-D1 Rat Novus Biologicals
NBP2-80656
RAW264.7 cells 100 µg/mL
CD81 Mouse Novus Biologicals
NBP2-53318
Gli36 cells 100 µg/mL
CD81* Mouse Novus Biologicals
NBP1-44861
Gli36 cells 100 µg/mL
CD83 Goat R&D Systems
AF2044
Dendritic cells 100 µg/mL
CD86 (B7-2) Goat R&D Systems
AF-141-NA
Dendritic cells 100 µg/mL
CD90 Mouse Abcam
ab23894
Cholangiocarcinoma cells 100 µg/mL
Collagen I Mouse Abcam
ab6308
(unpublished) 100 µg/mL
CPEB4 Rabbit Abcam
ab1974928
(unpublished) 100 µg/mL
CPT1A Mouse Abcam
ab128568
(unpublished) 100 µg/mL
CRBN Rabbit Novus Biologicals
NBP1-91810
HeLa cells 100 µg/mL
CYBRD1 Rabbit Genetex
GTX104793
Red blood cells 100 µg/mL
Cyclin A2 Rabbit Abcam
ab181591
(unpublished) 100 µg/mL
Cyclin D1 Rabbit R&D Systems
AF4196
MCF-7 cells 100 µg/mL
Cyclophilin B Goat R&D Systems
AF5410
HEK293T cells 100 µg/mL
Cytochrome b reductase 1 Rabbit Novus Biologicals
NBP2-16075
Red blood cells 100 µg/mL

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