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Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.
If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.
Single-Cell Western Validated Antibodies
| Validated Target | Host Species | Vendor / Cat # | Sample▲ | Concentration |
|---|---|---|---|---|
| CRMP5 | Rat |
Novus Biologicals NB100-74395 |
1:10 | |
| Histone H3 dimethyl lys 79 | Rabbit |
Novus Biologicals NBP2-59194 |
||
| IGF2BP3 | Rabbit |
EMD Millipore 03-198 |
100 µg/mL | |
| Myosin Light Chain 2 (MLC2) | Mouse |
Novus Biologicals NBP1-28871 |
1:10 | |
| mCherry | Mouse |
Novus Biologicals NBP2-43769 |
(mCherry)-HEK293T | 100 µg/mL |
| mCherry | Mouse |
Novus Biologicals NBP1-96752 |
(mCherry)-HEK293T | 100 µg/mL |
| mCherry | Rat |
ThermoFisher Scientific M11217 |
(mCherry)-HEK293T | 100 µg/mL |
| ALDH1A1 | Rabbit |
Cell Signaling 12035 |
(unpublished) | 1:10 |
| ATM | Mouse |
Novus Biologicals NB100-309 |
(unpublished) | 100 µg/mL |
| CD11b | Rabbit |
Novus Biologicals NB110-89474 |
(unpublished) | 1:10 |
| CLIP170 | Mouse |
Santa Cruz Biotechnology sc-166801 |
(unpublished) | 40 µg/mL |
| CLIP170 | Mouse |
Santa Cruz Biotechnology sc-28325 |
(unpublished) | 40 µg/mL |
| CLIP170* | Rabbit |
Cell Signaling 8977 |
(unpublished) | 1:10 |
| Collagen I | Mouse |
Abcam ab6308 |
(unpublished) | 100 µg/mL |
| CPEB4 | Rabbit |
Abcam ab1974928 |
(unpublished) | 100 µg/mL |
| CPT1A | Mouse |
Abcam ab128568 |
(unpublished) | 100 µg/mL |
| CRMP5 | Rat |
ThermoFisher Scientific MA3-701 |
(unpublished) | 1:10 |
| Cyclin A2 | Rabbit |
Abcam ab181591 |
(unpublished) | 100 µg/mL |
| Cytokeratin 14 | Rabbit |
ThermoFisher Scientific LBVRB-9020-P0 |
(unpublished) | 1:20 |
| Cytokeratin 19 | Rat |
Developmental Studies Hybridoma Bank TROMA III |
(unpublished) | 50 µg/mL |
| DDX4 | Rabbit |
Abcam ab13840 |
(unpublished) | 100 µg/mL |
| EDNRA | Mouse |
BD Biosciences 611252 |
(unpublished) | 1:20 |
| EpCAM | Rabbit |
Abcam ab213500 |
(unpublished) | 100 µg/mL |
| EpCAM* | Goat |
R&D Systems AF960 |
(unpublished) | 100 µg/mL |
| FGFR1 | Rabbit |
Cell Signaling 9740 |
(unpublished) | 1:10 |
| Glucagon | Mouse |
EMD Millipore MABN238 |
(unpublished) | 1:10 |
| Glucagon | Rabbit |
Abcam ab108426 |
(unpublished) | 100 µg/mL |
| Glucagon | Mouse |
BD Biosciences 565859 |
(unpublished) | 100 µg/mL |
| GSK3b | Mouse |
Cell Signaling 9832 |
(unpublished) | 1:10 |
| GST | Rabbit |
Cell Signaling 2625 |
(unpublished) | 1:10 |
| Hes1 | Rabbit |
Aviva Systems ARP32372-T100 |
(unpublished) | 1:5 |
| HMGCS2 | Rabbit |
Sigma Aldrich SAB2107997 |
(unpublished) | 100 µg/mL |
| HMGCS2* | Rabbit |
Abcam ab137043 |
(unpublished) | 100 µg/mL |
| IGFBP2 | Rabbit |
Abcam ab91404 |
(unpublished) | 100 µg/mL |
| IL-18 | Rabbit |
Abcam ab207324 |
(unpublished) | 1:10 |
| Insulin | Guinea Pig |
EMD Millipore AB3440 |
(unpublished) | 1:20 |
| Insulin* | Guinea Pig |
Dako A0564 |
(unpublished) | 1:40 |
| mlc2a | Rabbit |
ThermoFisher Scientific PA530789 |
(unpublished) | 1:10 |
| mlc2v | Mouse |
Promab 20324 |
(unpublished) | 1:10 |
| NEF | Mouse |
EMD Millipore MAB899 |
(unpublished) | 1:5 |
| NEF | Mouse |
Abcam ab42358 |
(unpublished) | 1:5 |
| Oct2 | Rabbit |
Novus Biologicals NBP1-89417 |
(unpublished) | 100 µg/mL |
| Oct2* | Rabbit |
Sigma Aldrich HPA008567 |
(unpublished) | 1:20 |
| Oct4 | Mouse |
Cell Signaling 75463 |
(unpublished) | 1:10 |
| Oct4* | Rabbit |
Cell Signaling 2750 |
(unpublished) | 1:10 |
| OLFM4 | Rabbit |
ThermoFisher Scientific PA5-73003 |
(unpublished) | 100 µg/mL |
| OLFM4* | Rabbit |
Novus Biologicals NBP2-24535 |
(unpublished) | 100 µg/mL |
| OMP* | Rabbit |
LifeSpan Biosciences, Inc. LS-C462178 |
(unpublished) | 1:10 |
| pGSK3β | Rabbit |
Abcam ab75814 |
(unpublished) | 100 µg/mL |
| pHistone H2A.X (Ser 139) | Rabbit |
Cell Signaling 9718 |
(unpublished) | 1:10 |
