Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies

Filter Antibodies: (394)

Validated Target	Host Species	Vendor / Cat #	Sample	Concentration
α-actinin	Goat	R&D Systems AF8279	KPC cells	100 µg/mL
α-actinin4	Mouse	Santa Cruz Biotechnology sc-390205	HeLa cells	100 µg/mL
α-synuclein	Rabbit	Cell Signaling 4179	SHY5Y cells	1:10
β-tubulin	Mouse	Genetex GTX11312	Primary Her2+ breast tumor cells	1:10
β-tubulin*	Rabbit	Abcam ab6046	Murine pancreatic cancer cells	1:40
β-tubulin*	Mouse	Genscript A-01717-40	Human pluripotent stem cells	1:40
βIII-tubulin (neuronal)	Mouse	Sigma Aldrich T8578	Human iPSCs	100 µg/mL
Actin	Mouse	Developmental Studies Hybridoma Bank 224-236-1	Dictyostelium discoideum	100 µg/mL
Akt (pan)	Mouse	Cell Signaling 2920	C2C12 cells	1:10
Akt (pan)	Rabbit	Cell Signaling 4685	C2C12 cells	1:10
Akt (pan)*	Rabbit	Cell Signaling 4691	C2C12 cells	1:10
Albumin	Mouse	Abcam 10241	Hepatocyte co-culture	100 µg/mL
Albumin	Rabbit	Cell Signaling 4929	Hepatocyte co-culture	1:10
ALDH1A1	Rabbit	Cell Signaling 12035	(unpublished)	1:10
alpha-actinin	Rabbit	Cell Signaling Technology 6487	BT474
AML1	Rabbit	Cell Signaling 4336	Jurkat cells	1:10
APRIL	Goat	R&D Systems AF884	MOLT-4 cells	1:5
ASC	Rabbit	Adipogen AG-25B-0006-C100	GM-CSF BMDCs	50 µg/mL
ATG5	Rabbit	Cell Signaling Technology CST129945	Macrophages	1:10
Atg7	Mouse	R&D Systems MAB6608	HeLa cells	100 µg/mL
ATG7	Mouse	R&D Systems MAB6608	Macrophages	1:10
Atg7*	Rabbit	Abcam ab133528	HeLa cells	100 µg/mL
ATM	Mouse	Novus Biologicals NB100-309	(unpublished)	100 µg/mL
ATP1A3	Mouse	Abcam ab2826	Live primary human cortical neurons	100 µg/mL
ATP1A3/Sodium Potassium ATPase Alpha 3	Mouse Monoclonal	Novus Biologicals NB300-540	Live primary human cortical neurons	100 µg/mL
Axl	Rabbit	Cell Signaling 8661	HeLa cells	1:10
BAFF	Goat	R&D Systems AF124	MOLT-4 cells	1:10
Bcl-xL	Rabbit	Cell Signaling 2764	HeLa cells	1:10
BCL6	Rabbit	Cell Signaling 5650	HeLa cells	1:10
BCL6	Rabbit	Cell Signaling 14895	HeLa cells	1:10
beta-tubulin	Mouse	Novus Biologicals NBP2-50061	Jurkat cells	50 µg/mL
beta-tubulin	Mouse	Novus Biologicals NB120-7792	Jurkat cells	50 µg/mL
betaIII-tubulin	Mouse	R&D Systems MAB1195	neural differentiated iPSCs	100 µg/mL
Blasticidin	Rabbit	Abcam ab38307	Transfected HEK293T cells	100 µg/mL
Blimp-1	Rabbit	Cell Signaling 9115	U266 cells	1:10
BRM	Rabbit	Cell Signaling 11966	293T cells	1:10
BTK	Rabbit	Cell Signaling 8547	Primary sorted human spleen lymphocytes	1:10
BTK	Mouse	Cell Signaling 56044	Primary sorted human spleen lymphocytes	1:10
c-Jun	Rabbit	Cell Signaling 9165	KPC cells	1:10
c-Myc	Rabbit	Cell Signaling 5605	Human pluripotent stem cells	1:10
Calnexin	Rabbit	Novus Biologicals NBP1-97476	U373 MG cells	1:10
Calnexin	Rabbit	Enzo Life Sciences ADI-SPA-865	U373 MG cells	1:10
Cas9	Mouse	Abcam ab191468	Mouse lymphocytes	1:10
Caspase 8	Mouse	Enzo ADI-AAM-118-E	Treated Jurkat cells	1:10
CCL3/MIP1	Goat	R&D Systems AF-270-NA	Differentiated THP-1 treated with LPS and Transport Inhibitors	20 µg/mL
CCR7	Rabbit	Novus Biologicals NBP2-58753	Jurkat cells	100 µg/mL
CD-80 (B7-1)	Goat	R&D Systems AF140	Dendritic cells	100 µg/mL
CD11b	Rabbit	Novus Biologicals NB110-89474	(unpublished)	1:10
CD19	Rabbit	Cell Signaling 3574	Ramos cells	1:10
CD209 (DC-SIGN)	Sheep	R&D Systems AF161	Dendritic cells	100 µg/mL
CD25	Goat	R&D Systems AF-223-NA	Tregs	100 µg/mL
CD29	Goat	R&D Systems AF2405	KPC cells	100 µg/mL
CD3	Rat	Cell Signaling 4443	MOLT-4 cells	1:10
CD3e	Rabbit	ThermoFisher Scientific MA5-14524	Differentiated NK cells	1:10
CD4	Goat	R&D Systems AF-379-NA	PBMCs	1:10
CD45	Mouse	BD Biosciences 555480	PBMCs	100 µg/mL
CD45	Rabbit	Cell Signaling 13917	PBMCs	1:10
CD56 (NCAM-1)	Goat	R&D Systems AF2408	Differentiated NK cells	100 µg/mL
CD68	Rat	Novus Biologicals NBP2-33337	RAW264.7 cells	100 µg/mL
CD68/SR-D1	Rat	Novus Biologicals NBP2-80656	RAW264.7 cells	100 µg/mL
CD8α	Rabbit	Cell Signaling 85336	CD3+ T-cells	1:10
CD81	Mouse	Novus Biologicals NBP2-53318	Gli36 cells	100 µg/mL
CD81*	Mouse	Novus Biologicals NBP1-44861	Gli36 cells	100 µg/mL
CD83	Goat	R&D Systems AF2044	Dendritic cells	100 µg/mL
CD86 (B7-2)	Goat	R&D Systems AF-141-NA	Dendritic cells	100 µg/mL
CD90	Mouse	Abcam ab23894	Cholangiocarcinoma cells	100 µg/mL
cFos	Mouse	Santa Cruz Biotechnology sc-166940	Treated LNCap cells	20 µg/mL
Cleaved caspase 8	Rabbit	Cell Signaling 9496	Treated Jurkat cells	1:10
CLIP170	Mouse	Santa Cruz Biotechnology sc-166801	(unpublished)	40 µg/mL
CLIP170	Mouse	Santa Cruz Biotechnology sc-28325	(unpublished)	40 µg/mL
CLIP170*	Rabbit	Cell Signaling 8977	(unpublished)	1:10
Collagen I	Mouse	Abcam ab6308	(unpublished)	100 µg/mL
Cortexillin I	Mouse	Developmental Studies Hybridoma Bank 241-438-1	Dictyostelium discoideum	1:2
CPEB4	Rabbit	Abcam ab1974928	(unpublished)	100 µg/mL
CPT1A	Mouse	Abcam ab128568	(unpublished)	100 µg/mL
CRBN	Rabbit	Novus Biologicals NBP1-91810	HeLa cells	100 µg/mL
CRBN	Rabbit	Cell Signaling Technology 71810	HeLa cells	1:10
CRMP5	Rat	Novus Biologicals NB100-74395		1:10
CRMP5	Rat	ThermoFisher Scientific MA3-701	(unpublished)	1:10
CYBRD1	Rabbit	Genetex GTX104793	Red blood cells	100 µg/mL
Cyclin A2	Rabbit	Abcam ab181591	(unpublished)	100 µg/mL
Cyclin D1	Rabbit	R&D Systems AF4196	MCF-7 cells	100 µg/mL
Cyclophilin B	Goat	R&D Systems AF5410	HEK293T cells	100 µg/mL
Cytochrome b reductase 1	Rabbit	Novus Biologicals NBP2-16075	Red blood cells	100 µg/mL
Cytokeratin 14	Mouse	ThermoFisher Scientific MA51599	normal human mammary basal cells	1:10
Cytokeratin 14	Rabbit	BioLegend 905301	Mammary tumor organoid cells	1:10
Cytokeratin 14	Rabbit	ThermoFisher Scientific LBVRB-9020-P0	(unpublished)	1:20
Cytokeratin 19	Rat	Developmental Studies Hybridoma Bank TROMA III	(unpublished)	50 µg/mL
Cytokeratin 19	Rabbit	GeneTex GTX112666	normal human mammary basal cells
Cytokeratin 8*	Mouse	Abcam ab9023	Breast cancer cells	100 µg/mL
Cytokeratin 8/18*	Rat	Developmental Studies Hybridoma Bank TROMA-1-S	Mammary tumor organoid cells	1:2
DDX4	Rabbit	Abcam ab13840	(unpublished)	100 µg/mL
DLL4	Goat	R&D Systems AF1389	KPC cells	100 µg/mL
DNMT3A	Goat	R&D Systems AF6315	SH-SY5Y	20 µg/mL
E-cadherin	Goat	R&D Systems AF748	KPC cells	100 µg/mL
EDNRA	Mouse	BD Biosciences 611252	(unpublished)	1:20
Efraim teams	Sheep	Whole sells products 1234567	Products all	1:30
EGFR	Mouse	Cell Signaling 2232	Circulating tumor cells	1:10
EGFR E746-A750	Rabbit	Cell Signaling 2085	HCC827 cells	1:10
EGFR L858R	Rabbit	Cell Signaling 3197	NCI-H1975 cells	1:10
EGFR*	Goat	R&D Systems AF231	BEAS-2B cells	100 µg/mL
EGR1	Rabbit	Abcam ab133695	Treated LNCap cells	1:20
eIF4E	Rabbit	Cell Signaling 2067	Primary Her2+ breast tumor cells	1:10
Eos	Rabbit	EMD Millipore ABE1331	HepG2 cells	100 µg/mL
EpCAM	Rabbit	Cell Signaling 3599	BT-20 cells	1:10
EpCAM	Rabbit	Abcam ab213500	(unpublished)	100 µg/mL
EpCAM*	Goat	R&D Systems AF960	(unpublished)	100 µg/mL
EphB4	Goat	R&D Systems AF446	Neural stem cells	1:20
ER	Rabbit	ThermoFisher Scientific RM-9101-S0	MCF-7 cells	1:10
ERa	Rabbit	Abcam ab16660	MCF-7 cells	1:10
ERK 1/2	Mouse	Cell Signaling 9107	A172 cells	1:10
ERK1/2	Rabbit	EMD Millipore 06-182	Neural stem cells	1:20
ERK1/2*	Mouse	Cell Signaling 4696	Stimulated PBMCs	1:10
ERK1/2*	Rabbit	Cell Signaling 4695	Primary Her2+ breast tumor cells	1:10
Etv5	Rabbit	Abcam ab156221	Pedx3-sorted cells	100 µg/mL
FAK	Sheep	R&D Systems AF4467	KPC cells	100 µg/mL
FGFR1	Rabbit	Cell Signaling 9740	(unpublished)	1:10
FGFR2	Rabbit	Cell Signaling 23328	KatoIII cells	1:10
FKBP5	Rabbit	Cell Signaling 12210	PBMCs	1:10
FLAG Tag	Rabbit	R&D Systems MAB8529	3T3 Flag-ATF6 cells	100 µg/mL
FoxL1	Rabbit	Abcam ab95286	A549 cells	1:10
FOXP3	Goat	R&D Systems BAF3240	Mouse T-regs
FOXP3	Rabbit	Novus Biologicals NB100-39002	FoxP3-transfected HEK293T	100 µg/mL
GAPDH	Goat	R&D Systems AF5718	HT-29 cells	100 µg/mL
GAPDH	Goat	Novus Biologicals NB300-320	Jurkat cells
GAPDH	Mouse	Novus Biologicals NB300-221	freshly isolated flow-sorted cells from skeletal muscle (Ecs, HCs, FAPS, MuSCs), Cells knocked down for TCF4 with siRNA at the level of transcript & protein	1:40
GAPDH	Goat	Everest EB06377	HeLa-GFP cells	100 µg/mL
GAPDH	Mouse	Genetex GTX627408	U373 MG cells	1:10
GAPDH	Rabbit	Cell Signaling 2118	HEK293 cells	1:20
GAPDH	Rabbit	Cell Signaling Technology 5174	HeLa cells	1:10
GAPDH	Mouse	EMD Millipore MAB374	HeLa cells	1:20
GAPDH*	Goat	Sigma Aldrich SAB2500450	A431 cells	1:10
GATA-3	Mouse	R&D Systems MAB6330	MCF-7 cells	100 µg/mL
GATA-3	Mouse	R&D Systems MAB26051	MCF-7 cells	100 µg/mL
GATA-3*	Goat	R&D Systems AF2605	MCF-7 cells	100 µg/mL
GATA-4	Rabbit	Cell Signaling 36966	Cardiomyocytes	1:10
GDNF	Goat	R&D Systems AF-212-NA	U-87 MG	20 µg/mL
GFAP	Sheep	R&D Systems AF2594	Human iPSCs	1:20
GFP	Mouse	Sigma Aldrich 11814460001	Primary murine germ cells	100 µg/mL
GFP	Rabbit	ThermoFisher Scientific A-11122	Dissociated mouse brain	100 µg/mL
GFP*	Goat	Abcam ab6673	Mouse dendrites	100 µg/mL
Glucagon	Mouse	EMD Millipore MABN238	(unpublished)	1:10
Glucagon	Rabbit	Abcam ab108426	(unpublished)	100 µg/mL
Glucagon	Mouse	BD Biosciences 565859	(unpublished)	100 µg/mL
Glucocorticoid Receptor	Rabbit	Cell Signaling 3660	PBMCs	1:10
GPCR MRGX2	Rabbit	Novus Biologicals NB110-75035	A549	1:10
GPR50	Rabbit	Cell Signaling 14032	293T cells	1:10
GRP75	Mouse	Abcam ab82591	U373 MG cells	1:10
GSK3b	Mouse	Cell Signaling 9832	(unpublished)	1:10
GST	Rabbit	Cell Signaling 2625	(unpublished)	1:10
H3K27me3	Mouse	Abcam ab205729	Human brain nuclei	100 µg/mL
H3K27me3	Mouse	Abcam ab6002	Treated KARPAS cells	100 µg/mL
H3K79me2	Rabbit	Novus Biologicals NBP2-59194	HeLa cells	100 µg/mL
H3K79me2*	Rabbit	Cell Signaling 5427	HEK293T cells	1:10
Helios	Rabbit	Cell Signaling 42427	PBMCs	1:10
Hemoglobin	Goat	Genetex GTX77484	Red blood cells	100 µg/mL
Hemoglobin*	Mouse	Rockland Immunochemicals 200-301-GS4	Red blood cells	1:10 (can lower to 1:20)
Her2	Mouse	Abcam ab16901	BT474
Her2	Mouse	ThermoFisher Scientific BMS120FI	BT474
Her2	Mouse	ThermoFisher Scientific MA513105	Primary Her2+ breast tumor cells	100 µg/mL
Hes1	Rabbit	Aviva Systems ARP32372-T100	(unpublished)	1:5
HIF1α	Mouse	BD Biosciences 610958	Treated HeLa cells	50 µg/mL
Histone H3	Rabbit	Novus Biologicals NB500-171	Jurkat cells
Histone H3	Mouse	Novus Biologicals NBP2-80770	PBMCs	100 µg/mL
Histone H3	Mouse	Cell Signaling 3638	PBMCS	1:10
Histone H3 dimethyl lys 79	Rabbit	Novus Biologicals NBP2-59194
Histone H3*	Sheep	Novus Biologicals NB100-747	Primary murine germ cells	100 µg/mL
Histone H3*	Mouse	Novus Biologicals NBP2-36468	PBMCs	100 µg/mL
Histone H3*	Rabbit	Cell Signaling 4499	CD4 T-cells	1:10
HMGB1	Mouse	R&D Systems MAB1690	HeLa cells	1:5
HMGCS2	Rabbit	Sigma Aldrich SAB2107997	(unpublished)	100 µg/mL
HMGCS2*	Rabbit	Abcam ab137043	(unpublished)	100 µg/mL
HSP60	Mouse	R&D Systems MAB1800	iPSCs	100 µg/mL
Iba1	Goat	Abcam ab48004	Dissociated mouse brain	100 µg/mL
IGF2BP3	Rabbit	EMD Millipore 03-198		100 µg/mL
IGFBP2	Rabbit	Abcam ab91404	(unpublished)	100 µg/mL
IgG	Rabbit	EMD Millipore AP101	IgG-producing cell line	1:40
Ikaros	Rabbit	Cell Signaling 14859	Jurkat cells	1:10
IL-18	Rabbit	Abcam ab207324	(unpublished)	1:10
IL-1beta	Goat	R&D Systems AF-401-NA	GM-CSF derived BMDCs	10 µg/mL
IL-2Rb	Goat	R&D Systems AF-224-NA	PBMCs	400 µg/mL
iNOS	Mouse	R&D Systems MAB9502	U2OS cells	1:10
Insulin	Guinea Pig	EMD Millipore AB3440	(unpublished)	1:20
Insulin*	Guinea Pig	Dako A0564	(unpublished)	1:40
IRF3	Mouse	Novus Biologicals NBP1-47812	THP1 cells	100 µg/mL
IRF3	Mouse	Novus Biologicals NBP1-04308	THP1 cells	100 µg/mL
IRF3*	Goat	R&D Systems AF4019	THP1 cells	100 µg/mL
IRF5	Goat	Abcam ab2932	THP1 cells	100 µg/mL
IRF5*	Sheep	R&D Systems AF4508	THP1 cells	100 µg/mL
K-Ras	Mouse	Santa Cruz Biotechnology sc-517599	A549	1:10
Lamin B1	Rabbit	R&D Systems AF8525	PBMCs	50 µg/mL
LANA	Rat	Abcam ab4103	Treated B-cells	100 µg/mL
LC3	Rabbit	Cell Signaling 12741	3T3-L1 cells	1:10
LC3	Rabbit	Sigma Aldrich L8918	3T3-L1 cells	100 µg/mL
Lck	Mouse	Cell Signaling 2657	Mouse T-regs	1:10
Lck*	Rabbit	Cell Signaling 2984	CD8 T-cells	1:10
Lin28b	Sheep	R&D Systems AF7726	K562 cells	1:10
Lin28b	Rabbit	Cell Signaling 4196	K562 cells	1:10
Lin28b	Rabbit	Cell Signaling 11965	K562 cells	1:10
Lin28b	Rabbit	Abcam ab191881	K562 cells

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