Milo Antibody Database

Find antibodies validated for Single-Cell Westerns on Milo

Milo is an open platform so you can use any Western-validated antibody to probe scWest chips. To help you get started, here is a selection of some of the antibodies that have been validated on Milo to date. This database is intended to provide general guidance in identifying and selecting antibodies to test. The performance of any antibody will need to be optimized for the specific sample type being studied. For more information on how to select antibodies for use on Milo, check out our tech note. Note: in cases where multiple antibodies have been validated for the same target, the best performing antibody is indicated by *. All primary antibodies have been incubated for 2 hours at room temperature. Listed sample types are not exhaustive.

If you don't see your antibody listed here, we can help! Please email us your target of interest at support@proteinsimple.com.

Single-Cell Western Validated Antibodies

Filter Antibodies: (394)
Validated Target Host Species▲ Vendor / Cat # Sample Concentration
α-actinin Goat R&D Systems
AF8279 		KPC cells 100 µg/mL
APRIL Goat R&D Systems
AF884 		MOLT-4 cells 1:5
BAFF Goat R&D Systems
AF124 		MOLT-4 cells 1:10
CCL3/MIP1 Goat R&D Systems
AF-270-NA 		Differentiated THP-1 treated with LPS and Transport Inhibitors 20 µg/mL
CD-80 (B7-1) Goat R&D Systems
AF140 		Dendritic cells 100 µg/mL
CD25 Goat R&D Systems
AF-223-NA 		Tregs 100 µg/mL
CD29 Goat R&D Systems
AF2405 		KPC cells 100 µg/mL
CD4 Goat R&D Systems
AF-379-NA 		PBMCs 1:10
CD56 (NCAM-1) Goat R&D Systems
AF2408 		Differentiated NK cells 100 µg/mL
CD83 Goat R&D Systems
AF2044 		Dendritic cells 100 µg/mL
CD86 (B7-2) Goat R&D Systems
AF-141-NA 		Dendritic cells 100 µg/mL
Cyclophilin B Goat R&D Systems
AF5410 		HEK293T cells 100 µg/mL
DLL4 Goat R&D Systems
AF1389 		KPC cells 100 µg/mL
DNMT3A Goat R&D Systems
AF6315 		SH-SY5Y 20 µg/mL
E-cadherin Goat R&D Systems
AF748 		KPC cells 100 µg/mL
EGFR* Goat R&D Systems
AF231 		BEAS-2B cells 100 µg/mL
EpCAM* Goat R&D Systems
AF960 		(unpublished) 100 µg/mL
EphB4 Goat R&D Systems
AF446 		Neural stem cells 1:20
FOXP3 Goat R&D Systems
BAF3240 		Mouse T-regs
GAPDH Goat R&D Systems
AF5718 		HT-29 cells 100 µg/mL
GAPDH Goat Novus Biologicals
NB300-320 		Jurkat cells
GAPDH Goat Everest
EB06377 		HeLa-GFP cells 100 µg/mL
GAPDH* Goat Sigma Aldrich
SAB2500450 		A431 cells 1:10
GATA-3* Goat R&D Systems
AF2605 		MCF-7 cells 100 µg/mL
GDNF Goat R&D Systems
AF-212-NA 		U-87 MG 20 µg/mL
GFP* Goat Abcam
ab6673 		Mouse dendrites 100 µg/mL
Hemoglobin Goat Genetex
GTX77484 		Red blood cells 100 µg/mL
Iba1 Goat Abcam
ab48004 		Dissociated mouse brain 100 µg/mL
IL-1beta Goat R&D Systems
AF-401-NA 		GM-CSF derived BMDCs 10 µg/mL
IL-2Rb Goat R&D Systems
AF-224-NA 		PBMCs 400 µg/mL
IRF3* Goat R&D Systems
AF4019 		THP1 cells 100 µg/mL
IRF5 Goat Abcam
ab2932 		THP1 cells 100 µg/mL
Musashi-1 Goat R&D Systems
AF2628 		Transfected Mesenchymal stem cells 100 µg/mL
N-Ras* Goat Abcam
ab77392 		Patient-derived bladder cancer cells implanted in nude mouse 1:10
Oct3-4 Goat R&D Systems
AF1759 		Embryonic stem cells 100 µg/mL
Olig2 (O2) Goat R&D Systems
AF2418 		25 day glial differentiated neural stem cells 100 µg/mL
OMP Goat Wako Chemicals
019-22291 		HepG2 cells 1:10
PAX2 Goat R&D Systems
AF3364 		AN3-CA 20 µg/mL
PAX3 Goat R&D Systems
AF2457 		U-87 MG 20 µg/mL
PD1 Goat R&D Systems
AF1086 		CD3+ T-cells 100 µg/mL
Smad1 Goat R&D Systems
AF2039 		KPC cells 100 µg/mL
Smad2/3 Goat R&D Systems
AF3797 		KPC cells 100 µg/mL
SOX1* Goat R&D Systems
AF3369 		iPSCs 100 µg/mL
Sox2 Goat Santa Cruz Biotechnology
sc-17320 		Neural stem cells 100 µg/mL
Sox2* Goat R&D Systems
AF2018 		NCCIT cells 100 µg/mL
Sox9* Goat R&D Systems
AF3075 		Primary murine germ cells 100 µg/mL
TGFbRII Goat R&D Systems
AF-241-NA 		HT1080 cells 100 µg/mL
TrkC Goat R&D Systems
AF1404 		CHO cells 1:2
VCAM1 Goat R&D Systems
AF643 		(unpublished) 100 µg/mL
Insulin Guinea Pig EMD Millipore
AB3440 		(unpublished) 1:20
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