Single Cell Protein Analysis with Milo

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Meet Milo

Milo™ performs Single Cell Western blot analysis on 1,000 single cells in one 4-hour experiment.

Single cell western blot consumables

Milo Consumables

The one-stop-shop for all the consumables you need to perform Single Cell Western blot analysis on Milo, including scWest kits, chips, and buffers.

Floating Antibody Cell

Milo Antibodies

Milo is an open platform so you can use any antibody to probe your target. Find antibodies validated for Milo.

The Single Cell Western Blot Has Arrived

Milo is the world’s first Single Cell Western blotting platform that measures protein expression in ~1000 single cells per run using conventional Western blot antibodies. Simply load your cell suspension on a scWest chip, then Milo performs lysis, SDS-PAGE separation, and UV-induced immobilization of each single cell lysate on-chip in a matter of minutes. Probe the scWest chip with conventional Western blot antibodies and use a microarray scanner to measure ~12 proteins per cell using a variety of multiplexing strategies, or store your chip for up to 9 months for future analysis. 

Request Single Cell Western Blot Pricing
Bioprocessing Cell Heterogeneity Protein Expression with Milo

Discover the World of Single Cell Proteomics with Milo

Here are just a few examples of the single cell analysis studies that are made possible with Single Cell Western blot analysis on Milo.

HIF1-a analysis with single cell western - Milo

Profile Heterogeneous Proteins and Cell Types

Milo’s single cell heterogeneity analysis profiles heterogeneous proteins and cell types in your samples that would go undetected by traditional Western blot.

Multiplexed Single Cell Heterogeneity Analysis of Single Red Blood Cells

Single Cell Heterogeneity Analysis of the Tumor Microenvironment 

6-well culture dish with different immune cell types in each well

Monitor Stem Cell Proteomics

Check out these App Notes to learn how Single Cell Western blotting with Milo can track and quantify single stem cell proteomics and differentiation into neural cell subtypes and cardiomyocytes:

Identify and Quantify Neural Cell Subtypes

Track iPSC to Cardiomyocyte Differentiation

Easier Than Single Cell Flow Cytometry

Milo is easier to use than other single cell analysis technologies like single cell flow cytometry. With Milo’s single cell analysis, you can detect proteins in single cells that escape detection by single cell flow cytometry due to their intracellular location or due to the lack of flow cytometry-validated antibodies. You can also pair Milo with fluorescence-activated cell sorting (FACS) to sort cell subtypes or live/dead cells for downstream single cell analysis on Milo.

Learn How Milo Streamlines Your Single Cell Flow Cytometry Experiments

Milo’s single cell protein analysis
Single-cell Western Cell and Gene Therapy - sickle cell analysis

Develop New Cell and Gene Therapies

Reveal the functional potency of viral vectors and other gene delivery systems. Measure their gene editing efficiency by monitoring target protein expression in single cells following genetic transformation to develop new cell and gene therapies.

Milo protein validation workflow of single-cell RNA seq data

Validate Single Cell Sequencing Data

Pair Milo’s single cell proteomics with single cell RNA sequencing workflows for complete single cell multiomic analysis. Milo provides single cell protein expression information to validate your single cell RNA seq analysis single cell protein expression data. 

Publish Your Single Cell Western Blots in Premier Journals

Milo has proven its ability to pro­vide new and profound scientific insight that traditional Western blots cannot. This fact is reflected in the rank of the journals that feature articles with Single Cell Western blot data with the vast majority (74%) of articles featuring Single Cell Western blot data in top journals with impact factors of 7 or greater including Science, Cell, Nature Methods, Nature Communications, and Cell Reports. 32% of Single Cell Western blot publications are in journals considered here to be in the premier category with an impact factor greater than 21.

Learn How Single Cell Western Blot is a Proven, High-Impact Technology

Protein Simple let's you meet scientific standards by delivering publication-ready results

Customer Success Stories

Requirements and Compatibility

Sample type

Suspension containing >10,000 cells

Cell diameter

7-25 µm in suspension

Cell type

Mammalian cells; globular in suspension and unfixed

Antibody requirement

Standard unlabeled primaries and fluorescent secondaries

Other equipment needed

Open-format fluorescence microarray scanner capable of 5 µm resolution

 

Performance and Specifications

Typical cell dilutions

Yield capture and analysis of 1,000-2,000 cells per scWest chip

Molecular weight (MW) range

15-175 kDa

MW resolution

10% difference in distinct spectral channels, as low as 30% differences in same spectral channel

Typical target multiplexing

Up to four proteins per cell by spectral and size-based multiplexing
Twelve-plus proteins per cell using stripping & reprobing

Workflow time

4-6 hours

Single Cell Western Blot Applications

immune cells attack cancer cell

Cancer and Immuno-Oncology

Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples.

Testing for Lentivirus and AAVs

Cell and Gene Therapy

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Solutions for Cell Signaling and Signal Pathways Analysis

Cell Signaling

Uncover phospho-protein heterogeneity and quantify how phosphorylation varies across stimulated and unstimulated cell populations.

Neuroscience App image for Ella

Neuroscience

Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.