Stellar Fluorescence and Chemiluminescence Immunoassay

Stellar™ Sets the Industry Standard for Western Blotting Fluorescence Detection Sensitivity

Stellar on Simple Western's Jess is a multiplex fluorescence immunoassay and chemiluminescence immunoassay

• Multiplex low abundance targets including total and phospho-protein isoforms alongside total protein normalization
• Low picogram protein NIR/IR detection sensitivity
• Simultaneous total protein normalization at low concentration ranges
• Superior signal linearity across a 4-log dynamic range
• Full automation of Western blot analysis plus flexible multiplex strategies

Stellar Boosts Fluorescence Detection Sensitivity for Your Fluorescence and Chemiluminescence Immunoassay

Stellar fluorescence detection is 100X more sensitive than traditional fluorescent western blots

• Stellar NIR/IR fluorescence detection offers comparable sensitivity to Simple Western™ chemiluminescence and higher sensitivity than competing traditional fluorescence western blotting approaches. 
• Stellar fluorescence immunoassay demonstrated 100X greater detection sensitivity than the closest competitive traditional Western blot fluorescence imaging system.

Right Figure and Table Below: Comparison of the limit of detection for RNase A on Jess using chemiluminescence immunoassay and Stellar NIR (red bands) and Stellar IR (green bands) detection modules with a leading competitor Western blot fluorescence imaging system.

Multiplex Fluorescence and Chemiluminescence Immunoassays with Total Protein Normalization

Accurate Protein Expression Measurements, Including Total and Phospho-Protein Isoforms

Multiplex multiple targets and perform the Stellar Total Protein Assay to normalize your fluorescence immunoassay signals for more accurate results – all in the same sample.

Right Figure: Lysates from Jurkat cells either untreated (-) or treated (+) with Calyculin A were probed for total AKT (IR, green bands) and phospho AKT (NIR, red bands). Each antibody was run alone and multiplexed (left panel) in both lysates along with a Stellar Total Protein Assay (right panel).

Note: Stellar Total Protein Detection Module requires the use of a Stellar NIR and/or IR Detection Module in the same run.

Multiplex Fluorescence and Chemiluminescence Immunoassays with Excellent Reproducibility

Stellar NIR/IR Detection channels enabled multiplexing total and phosphorylated AKT in the same lane with excellent reproducibility.

Right Figure: Jurkat whole-cell lysate probed using mouse anti-total AKT and rabbit anti-phospho AKT primary antibodies and Stellar Mouse IR (green bands) and Stellar Rabbit NIR (red bands) Detection Modules. <10% CVs for pAKT and <5% CVs for Total AKT across 24 lanes

Multiplex with High Efficiency and Get Stellar Results in 3 Hours

Dramatically Reduce Time to Results Compared to Traditional Western Blotting Workflows

Stellar NIR/IR detection modules reduce your time to data compared to RePlex™ and traditional Western blot when multiplexing with antibodies of the same species and/or antibody targets of similar size

• Both Stellar NIR/IR and RePlex assays detected total-AKT and phospho-AKT targets within the same lane.
• Stellar NIR/IR detection runs were completed in approximately 3 hours compared to the 5-hour run time of RePlex
• Both Stellar and RePlex dramatically speed up time to multi-target data when compared to traditional Western blotting workflows