Testimonials From Your Peers

Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform.

Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots.

Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease

The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy.

It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. The appropriate control and low variability between plates using Simple Western™ means we can complete our studies in weeks rather than months.

We needed assays that were not only excellent at conserving our limited sample volumes but would allow us to test a lot of markers quickly and efficiently. The Luminex and Simple Plex assays proved to be the ideal choice in this case, giving us a level of accuracy and reproducibility that we’ve been unable to get with other platforms.

We were fortunate to be able to test these Luminex porcine panels early in our research effort. Once we could see the measurements we were getting on the plates, we knew we had found what we were looking for. The panels ended up making a huge difference in the outcome of the study.

I was looking at two different isoforms of a protein, and it was important to know if the cells were expressing just one versus the other or both in a given cell. That was tricky until Milo came. We’re also getting more relevant information because Milo allows us to look at expression of this protein in tissue biopsies.

Milo permits study of proteins at the single-cell level to understand the heterogeneity among metastatic cells within a population.

We have been pairing Milo with single-cell real time qPCR data. We have the RNA and we know the transcripts are present. We used Milo to confirm our results and quantify the amount of protein that is in each individual cell.

One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. We were spending more than 4 hours of hands-on time per Western blot with traditional methods, and we can set up a run on the Wes in about 45 minutes. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods.

We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. With Wes, we can do multiple assays with one sample collection.