Testimonials From Your Peers

The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies.

We had been trying to study receptor proteins with traditional Westerns but it was frustrating—we just could not get it. A few months with Wes and we've already had way more success than we had in three years.

The traditional blotting method can use up to 300 pieces of plastic and [Simple Western] uses two. What's even better is that [Simple Western] can run up to 40 proteins in three days. It would take about a week to run 40 proteins using Western blotting.

The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-Iα and PKG-Iβ and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies.

Wes gives me more throughput so I can get more replicates and trust my results a little bit more, and I can trust them straight away rather than having to do a lot of repeats. And obviously I like getting results the same day rather than having to wait two days

The no-gel, no-transfer, no-membrane features are beneficial for the reproducibility of the assay. I really like this system over traditional Western.

We’ve been able to increase productivity dramatically with Wes. The results are much better too, given that it removes the blotting step all together.

[Simple Western] is easy to use, convenient, and gives answers quickly. The throughput and system automation is a definite advantage.

Some of the proteins we wanted to detect are low in abundance and it’s really hard to get a good amount of protein for traditional Western blot. The low protein concentrations required by [Simple Western] makes it easier to save precious samples ensuring protein detection.

Wes allows us to measure cell signaling proteins from a single muscle fiber segment by lowering our limit of detection nearly ~20-fold from Western blotting. It also allows for much more precise, reproducible, and faster results to be generated, in addition to far greater ease of operation, analysis, and waste disposal.

[Wes] Provides quantitative data, saves time and uses far less sample.

I was able to screen various antibodies in a single run within half a day. This had previously taken weeks and months using traditional Western blotting methods