trans-ISRIB

Name: trans-ISRIB
Brand: Tocris Bioscience
SKU: 5284
Rating: 5 (2 reviews)

Catalog # 5284 | Tocris Bioscience a Bio-Techne Brand

(2)

Citations (13)

COA / SDS

Integrated stress response (ISR) inhibitor

Key Product Details

Description:	Integrated stress response (ISR) inhibitor
Chemical Name:	N,N'-trans-1,4-cyclohexanediylbis[2-(4-chlorophenoxy)acetamide]
Purity:	≥99% (HPLC)
Molecular Weight:	451.34

View Full Technical Details for trans-ISRIB

Technical Data
Citations (13) / Reviews (2)

Biological Activity for trans-ISRIB

trans-ISRIB is a potent integrated stress response (ISR) inhibitor; reverses the effects of eIF2α phosphorylation (IC₅₀ = 5 nM) and restores cell translation capacity. Acts downstream of PERK, targeting interactions between eIF2α kinases and elF2B. Blocks ATF4 production without altering the IRE1 or ATF6 responses in vitro. Displays memory enhancement in hippocampus-dependent spatial and fear-associated learning in rodents. trans-ISRIB promotes survival of pluripotent stem cells in culture when used in combination with Chroman 1 (Cat. No. 7163), Emricasan (Cat. No. 7310), and Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), a combination known as CEPT. When used as part of the CEPT cocktail, trans-ISRIB improves survival of differentiated cells following cryopreservation. Brain penetrant.

Technical Data for trans-ISRIB

M. Wt	451.34
Formula	C₂₂H₂₄Cl₂N₂O₄
Storage	Store at +4°C
Purity	≥99% (HPLC)
CAS Number	1597403-47-8
PubChem ID	1011240
InChi Key	HJGMCDHQPXTGAV-UHFFFAOYSA-N
Smiles	O=C(COC1=CC=C(Cl)C=C1)N[C@@H]2CC[C@@H](NC(COC3=CC=C(Cl)C=C3)=O)CC2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for trans-ISRIB

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	4.51	10 with gentle warming

Preparing Stock Solutions for trans-ISRIB

The following data is based on the product molecular weight 451.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	22.16 mL	110.78 mL	221.56 mL
0.5 mM	4.43 mL	22.16 mL	44.31 mL
1 mM	2.22 mL	11.08 mL	22.16 mL
5 mM	0.44 mL	2.22 mL	4.43 mL

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References for trans-ISRIB

References are publications that support the biological activity of the product.

Chen A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 2021 PMID: 33941937
Sekine Stress responses. Mutations in a translation initiation factor identify the target of a memory-enhancing compound. Science 2015 PMID: 25858979
Hinnebusch Cell biology. Blocking stress response for better memory? Science 2015 PMID: 26023119
Sidrauski Pharmacological brake-release of mRNA translation enhances cognitive memory. Elife 2013 PMID: 23741617

Citations for trans-ISRIB (13)

Showing 1 - 10 of 13 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for trans-ISRIB include:

Lisa D et al. (2023) A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas. Cancer Discov. 13:766-795
PMID: 36576405
Jeffrey D et al. (2022) Polyadenylated RNA and RNA-Binding Proteins Exhibit Unique Response to Hyperosmotic Stress. Front Cell Dev Biol. 9:809859
PMID: 34970554
Jason A et al. (2022) Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Nat Commun. 13:3244
PMID: 35688816
Jing et al. (2022) Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR. Cell Rep. 40:111092
PMID: 35858571
Tristan et al. (2021) Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. Stem Cell Rep.. 16:3076
PMID: 34861164
Bo et al. (2021) SARS-CoV-2 infection induces beta cell transdifferentiation. Cell Metab. 33:1577-1591.e7
PMID: 34081913
Viviana et al. (2021) Cholinergic neurons constitutively engage the ISR for dopamine modulation and skill learning in mice. Science. 372
PMID: 33888613
Jacques et al. (2020) Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway. Nutrients. 12
PMID: 33302552
Chen et al. (2019) A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods. 18:528
PMID: 33941937
Gary W et al. (2019) Adaptive Protein Translation by the Integrated Stress Response Maintains the Proliferative and Migratory Capacity of Lung Adenocarcinoma Cells. Mol Cancer Res. 17:2343-2355
PMID: 31551255
Victor X et al. (2019) The eIF2α Kinase GCN2 Modulates Period and Rhythmicity of the Circadian Clock by Translational Control of Atf4. Neuron. 104:724-735.e6
PMID: 31522764
Peter et al. (2018) Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate. Mol Cell. 71:629-636.e5
PMID: 30118681
Krokowski (2017) GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep.. 21(10):2895
PMID: 29212034

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Customer Reviews for trans-ISRIB (2)

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Inhibits integrated stress response in HEK293 cells

Name: mandi gandelman

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: HEK293

Used trans-ISRIB to inhibit the integrated stress response (ISR) in HEK293 cells. Induced the ISR and added ISRIB (100 and 500nM) overnight. Evaluated p-eif2a (unchanged) and cleaved caspase 3 (increased in a dose-dependent manner with ISRIB.
High specific inhibitor

Name: Anonymous

Species: Rat

Assay Type: In Vitro

Cell Line/Tissue: cortical neurons

Toxicity assay. 500 nM concentration was used. Highly effective Integrated stress response (ISR) inhibitor. Works very well.

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