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Antibodies for Western Blot

Primary Antibodies

Bio-Techne® offers a large portfolio of primary antibodies validated for Western blot experiments.

Secondary Antibodies

Bio-Techne offers enzyme- and fluorochrome-conjugated secondary antibodies for use in Western blot experiments.

Epitope Tag Antibodies

Bio-Techne offers antibodies for the most commonly used epitope tags for the detection of tagged proteins with Western blot.

Novus Biologicals Western Blot Tools

Novus Biologicals offers reagents for your entire Western blot workflow including over 65,000 Western blot-validated antibodies, HRP-conjugated secondary antibodies, detection reagents, and controls such as blocking peptides, control lysates, and over-expression lysates.

Simple Western™ Automated Western Blotting Systems

Western blots are notoriously labor-intensive and challenging to perform. To overcome the challenges of traditional Western blots, Simple Western™ from ProteinSimple, a Bio-Techne brand, offers fully automated capillary Western blot analysis. The steps traditionally performed in Western blotting techniques like separation, immobilization, washing, and detection are automatically performed inside a benchtop capillary electrophoresis machine.

  • Simple Western offers high throughput Western blot analysis with fully quantitative results on up to 25 samples in just 3 hours, or 96 samples overnight.
  • Simple Western users enjoy industry-leading NIR/IR fluorescence detection sensitivity with Stellar™ Modules on Jess™.
  • Simple Western even replaces the laborious strip and re-probe of traditional Western blot testing with RePlex™, which removes the antibodies from the first round of probing for a second round of probing with fresh antibodies or total protein normalization.
Simple Western on Jess vs. traditional Western blot
Jess Simple Western instrument by ProteinSimple, a Bio-Techne brand


Jess is an automated capillary-based Western blotting system. Jess’s capabilities include chemiluminescence and two-color fluorescence detection with industry-leading sensitivity, and built-in total protein normalization. Jess is compatible with Stellar NIR/IR and RePlex modules.

Abby Simple Western instrument by ProteinSimple, a Bio-Techne brand


Abby is an automated capillary-based Western blotting system. Abby can process up to 25 samples in just 3 hours and it comes with sensitive chemiluminescence detection. Abby is compatible with the RePlex module for sequential immunoassays or total protein normalization.

Sally Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Sally Sue

Are you looking for high throughput Western blot testing? Sally Sue lets you separate and analyze proteins by size in a fully automated fashion. Process up to 96 sample overnight, and get up to 8 data points on just 5 µL of sample.

Peggy Sue Simple Western instrument ProteinSimple, a Bio-Techne brand

Peggy Sue

Bio-Techne’s Peggy Sue offers capillary Western blot analysis that lets you separate proteins by either size or charge in an automated fashion. Only 5 µL of sample is needed, and up to 96 samples can be processed overnight for high throughput Western blot analysis.

Nanopro 1000 Simple Western instrument ProteinSimple, a Bio-Techne brand

NanoPro 1000

The NanoPro 1000 is Bio-Techne’s automated capillary Western blot platform that uses isoelectric focusing to characterize proteins by charge. Get rich charge heterogeneity information to reveal post-translational modifications and other protein isoforms. Process up to 96 samples overnight for high throughput Western blot analysis.

Compass Software

Compass for Simple Western Software

Analyze Simple Western Size or Charge assay data with Bio-Techne’s Compass for Simple Western software. Equipped with features that satisfy the 21 CFR Part 11 data security requirements.

Now You Can Perform Western Blotting in Single Cells with Milo™

Are you interested in single-cell proteomics? Milo™ is our Single-Cell Western blotting technique that measures protein expression in ~1,000 single cells in a single run. Validate your RNAseq data, analyze FACS-sorted cells, and gain a rich understanding of single-cell proteomics with Milo, the World’s first Single-Cell Western blotting technique.

Related Western Blot Resources

Western Blot Handbook

This step-by-step Western blot guide from Novus Biologicals is a great introduction to Western blot. It provides detailed information for understanding, performing, and troubleshooting a Western blot protocol.

Western Blot Troubleshooting Resource

A comprehensive, online resource for troubleshooting the most common problems of Western blot including no signal, multiple bands, and high background.

Development of Validated Monoclonal Antibodies

This white paper describes our validation process, including antigen design, feasibility testing, specificity and sensitivity testing, and quality control and release decisions, for our monoclonal antibodies.

Background Information